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Cellulase production and its localization inTrichoderma harzianum

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Abstract

The ability ofT. harzianum to produce the three components of cellulase,viz. filter paper activity, carboxymethylcellulase and β-glucosidase was investigated. The optimum pH and temperature for all the enzymes in the culture nitrate was 5–7 and 27°C, respectively. The shaken culture conditions gave low yields of the enzyme as compared to static cultures. Most of the FP-activity and CM-cellulase were located extracellularly but reasonable amount of β-glucosidase was retained in the cell debris fraction.

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Authors and Affiliations

  1. Department of Biology, Guru Nanak Dev University, 143 005, Amritsar, India

    M. K. KalRa & D. K. Sandhu

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  1. M. K. KalRa
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  2. D. K. Sandhu
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The authors acknowledge the receipt of fellowship from theCouncil of Scientific and Industrial Research, New Delhi.

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KalRa, M.K., Sandhu, D.K. Cellulase production and its localization inTrichoderma harzianum . Folia Microbiol 31, 303–308 (1986). https://doi.org/10.1007/BF02926955

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  • DOI: https://doi.org/10.1007/BF02926955

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