Summary
Production and release of cellulolytic enzymes by Trichoderma reesei QM 9414 were studied under induced and non-induced conditions. For that purpose, a method was developmed to produce cellulases by Trichoderma reesei QM 9414 using the soluble inducer, cellobiose, as the only carbon source. The production was based on continuous feeding of cellobiose to a batch culture. For optimum production, the cellobiose supply had to be adjusted according to the consumption so that cellobiose was not accumulated in the culture. With a proper feeding program the repression and/or inactivation by cellobiose could be avoided and the cellulase production by Trichoderma reesei QM 9414 was at least equally as high as with cellulose as the carbon source.
During the cultivation, specific activities against filter paper, carboxymethyl cellulose (CMC) and p-nitrophenyl glucoside were analyzed from the culture medium as well as from the cytosol and the cell debris fractions. There was a base level of cell debris bound hydrolytic activity against filter paper and p-nitrophenyl glucoside even in T. reesei grown non-induced on glycerol. T. reesei grown on cellobiose was induced to produce large amounts of extracellular filter paper and CMC hydrolyzing enzymes, which were actively released into the medium even in the early stages of cultivation. β-Glucosidase was mainly detected in the cell debris and was not released unless the cells were autolyzing.
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References
Berg B, Pettersson G (1977) J Appl Bacteriol 42:65–75
Berg B (1978) Arch Microbiol 118:61–65
Berghem LER, Pettersson LG (1973) Eur J Biochem 37:21–30
Binder A, Ghose TK (1978) Biotechnol Bioeng 20:1187–1199
Brown DE (1976) Biotechnol Bioeng Symp 6:75–77
Eriksson K-E, Hamp SG (1978) Eur J Biochem 90:183–190
Ghose TK, Pathak AN, Bisaria VS (1975) Symposium on enzymatic hydrolysis of cellulose, Bailey M, Enari T-M, Linko M (eds). Aulanko, Finland, pp 111–136
Herr D, Luck G, Dellweg H (1978) J Ferment Technol 56:273–278
Hunter MJ, Commerford SL (1961) Biochem Biophys Acta 47:580–586
Hägerdal BGR, Ferchak JD, Pye EK (1978) Appl Environ Microbiol 36:606–612
Mandels M, Andreotti RE (1978) Process Biochem 13:6–13
Mandels M, Reese ET (1957) J Bacteriol 73:269–278
Mandels M, Reese ET (1960) J Bacteriol 79:816–826
Mandels M, Weber J (1969) Adv Chem Ser 95:391–413
Miller LG (1959) Anal Chem 31:426–428
Moreira AR, Phillips JA, Humphrey AE (1978) Biotechnol Bioeng 20:1501–1505
Nisizawa T, Suzuki H, Nakayama M, Nisizawa K (1971) J Biochem 70:375–385
Peitersen N, Medeiros J, Mandels M (1977) Biotechnol Bioeng 19:1091–1094
Pollock MR (1962) Exoenzymes. In: Gunsalus IC, Stainer RY (eds) The Bacteria, Vol 4. Academic Press, New York, pp 121–178
Simmons EG (1977) Second International Mycological Congress, Tampa Florida, p 618
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Vaheri, M.P., Vaheri, M.E.O. & Kauppinen, V.S. Formation and release of cellulolytic enzymes during growth of Trichoderma reesei on cellobiose and glycerol. European J. Appl. Microbiol. Biotechnol. 8, 73–80 (1979). https://doi.org/10.1007/BF00510268
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DOI: https://doi.org/10.1007/BF00510268