Summary
Carboxymethyl-cellulase and β-glucosidase activities were determined in the cytosole, cell walls and extracellular culture fluid of Trichoderma reesei QM 9414 cultivated on cellulose and cellobiose. By means of carboxymethylcellulose as a specific desorbens for cellulose bound CM-cellulase and β-glucosidase it was found that these enzymes are cell wall bound during consumption of the carbon source, but are excreted during the subsequent cultivation stage. Treatment of intact cell walls with various chemical agents could not cause a release of the enzyme. Treatment of intact cell walls with α-mannanase or trypsin released CM-cellulase, whereas, treatment with laminarinase or chitinase released β-glucosidase. Both enzymes were also released during autolysis in phosphate buffer. This autolysis was accompanined by the appearance of extracellular mannanase, laminarinase and proteinase. The results suggest that cleavage of chemical bonds of certain cell wall polymers of T. reesei could be responsible for the appearance of CM-cellulase and β-glucosidase in the culture fluid during later stages of growth.
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Kubicek, C.P. Release of carboxymethyl-cellulase and β-glucosidase from cell walls of Trichoderma reesei . European J. Appl. Microbiol. Biotechnol. 13, 226–231 (1981). https://doi.org/10.1007/BF00500103
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DOI: https://doi.org/10.1007/BF00500103