Summary
Human heart-type cytoplasmic fatty acid-binding protein (HH-FABPc) has been proposed as an early biochemical indicator of acute myocardial infarction (AMI). However, skeletal muscles also contain HH-FABPc identical to that found in the heart. Before HH-FABPc can be clinically employed as an indicator of AMI, its content in various tissues other than the heart must be known. Accordingly, we measured the HH-FABPc content of various human muscles and organs, using a sandwich enzyme-linked immunosorbent assay (ELISA) for HH-FABPc. HH-FABPc was abundant in the ventricles (0.46 mg/g wet weight and 1.5% of the cytoplasmic protein in the left ventricle), while the atria contained slightly less HH-FABPc (0.25 mg/g wet weight and 0.7% of the cytoplasmic protein in the left atrium). Of the skeletal muscles tested, the diaphragm contained about one-quarter of the HH-FABPc content of the heart, but other skeletal muscles contained very low levels of this protein. Other than the muscles, the kidneys contained less than one-tenth of the HH-FABPc in the heart, and negligible amounts were found in the liver and small intestine. The distribution of HH-FABPc in the heart and skeletal muscles was comparable to that of cardiac-specific creatine kinase (CK-MB) activity, and was inverse to the distribution of myoglobin. The plasma myoglobin/HH-FABPc ratio, determined in patients with AMI and those without AMI, closely reflected that in the heart and skeletal muscles. These findings indicate that HH-FABPc may be useful as a specific indicator of AMI, and the plasma myoglobin/HH-FABPc ratio could provide valuable information for the diagnosis of AMI.
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References
Glatz JFC, Van Bilsen M, Paulussen RJA, Veerkamp JH, Van der Vusse GJ, Reneman RS (1988) Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox. Biochim Biophys Acta 961:148–152.
Knowlton AA, Apstein CS, Saouf R, Brecher P (1989) Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat. J Mol Cell Cardiol 21:577–583
Knowlton AA, Burrier RE, Brecher P (1989) Rabbit heart fatty acid-binding protein. Isolation, characterization, and application of a monoclonal antibody. Circ Res 165:981–988
Tanaka T, Hirota Y, Sohmiya K, Nishimura S, Kawamura K (1991) Serum and urinary human fatty acid-binding protein in acute myocardial infarction. Clin Biochem 24:195–201
Kleine AH, Glatz JFC, Van Nieuwenhoven FA, Van der Vusse GJ (1992) Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in man. Mol Cell Biochem 116:155–162
Tsuji R, Tanaka T, Sohmiya K, Hirota Y, Yoshimoto K, Konoshita K, Kusaka Y, Kawamura K, Morita H, Abe S, Tanaka H (1993) Human heart-type cytoplasmic fatty acid-binding protein in serum and urine during hyperacute myocardial infarction. Int J Cardiol 41:209–217
Sohmiya K, Tanaka T, Tsuji R, Yoshimoto K, Nakayama Y, Hirota Y, Kawamura K, Matsunaga Y, Nishimura S, Miyazaki H (1993) Plasma and urinary heart-type cytoplasmic fatty acid-binding protein in coronary occlusion and reperfusion-induced myocardial injury model. J Mol Cell Cardiol 25:1413–1426
Glatz JFC, Kleine AH, Van Nieuwenhoven FA, Hermens WT, Van Dieijen-Viser MP, Van der Vusse GJ (1994) Fatty-acid-binding protein as a plasma marker for the estimation of myocardial infarct size in humans. Br Heart J 71:135–140
Puleo PR, Roberts R (1988) An update on cardiac enzymes. Cardiol Clin 6:97–109
Peeters RA, In’t Groen MA, Veerkamp JH (1989) The fatty acid-binding protein from human skeletal muscle. Arch Biochem Biophys 274:556–563
Ohkaru Y, Asayama K, Ishii H, Nishimura S, Sunahara N, Tanaka T, Kawamura K (1995) Development of a sandwich enzyme-linked immunosorbent assay for the determination of human heart fatty acid-binding protein in plasma and urine by using two different monoclonal antibodies specific for human heart fatty acid-binding protein. J Immunol Methods 178:99–111
Hangaard J, Rasmussen O, Norgaard Hanssen K, Jorgensen N, Simonsen EF, Norgaaed-Pedersen B (1987) Early diagnosis of acute myocardial infarction with a rapid latex agglutination test for semi-quantitative estimation of serum myoglobin. Acta Med Scand 221: 343–348
Mair J, Artner-Dworzak E, Lechleitner P, Morras B, Smidt J, Wagner I, Dienstl F, Puschendorf B (1992) Early diagnosis of acute myocardial infarction by a newly developed rapid immunoturbidimetric assay for myoglobin. Br Heart J 68:462–468
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Tanaka T, Hosaka K, Hoshimaru M, Numa S (1979) Purification and properties of long-chain acyl-coenzyme A synthetase from rat liver. Eur J Biochem 98:165–172
Gruppo Italiano per lo Studio della Streptochinasi nell’Infarto Miocardico (GISSI) (1986) Effectiveness of intravenous thrombolytic treatment in acute myocardial infarction. Lancet (Feb 22):397–402
Simoons ML, Serruys PW, Van den Brand M, Res J, Verheugt FWA, Krauss XH, Remme WJ, Bär F, De Zwaan C, Van der Laarse A, Vermeer F, Lubsen J (1986) Early thrombolysis in acute myocardial infarction: Limitation of infarct size and improved survival. J Am Coll Cardiol 7:717–728
Van der Werf F, Arnold AER (1988) Intravenous tissue plasminogen activator and size of infarct, left ventricular function, and survival in acute myocardial infarction. Br Med J 297:1374–1379
ISIS-2 (Second International Study of Infarct Survival) Collaborative Group (1988) Randomized trial of intravenous streptokinase, oral aspirin, both, or neither among 17,187 cases of suspected acute myocardial infarction: ISIS-2. Lancet (Aug 13):342–360
Bang NU, Whilhelm OG, Clayman MD (1989) After coronary thrombolysis and reperfusion, What next? J Am Coll Cardiol 14:837–849
Braunwald E (1989) Myocardial reperfusion, limitation of infarct size, reduction of left ventricular dysfunction, and improved survival. Should the paradigm be expanded? Circulation 79:441–444
Paulussen RJA, van Moerkerk HTB, Veerkamp JH (1990) Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues. Int J Biochem 22: 393–398
Crisman TS, Claffey KB, Saouaf R, Hanspal J, Brecher P (1987) Measurement of rat heart fatty acid binding protein by ELISA. Tissue distribution, developmental changes and subcellular diatribution. J Mol Cell Cardiol 19:423–431
Vork MM, Glatz JFC, Surtel DAM, Knubben HJM, Van der Vusse GJ (1991) A sandwich enzyme-linked immunosorbent assay for the determination of rat heart fatty acid-binding protein using the streptavidin-biotin system. Application to tissue and effluent samples from normoxic rat heart perfusion. Biochim Biophys Acta 1075:199–205
Ellis AK, Little T, Masud AR, Liberman HA, Moris DC, Klocke FJ (1988) Early noninvasive detection of successful reperfusion in patients with acute myocardial infarction. Circulation 1988:1352–1357
Katus HA, Diederich KW, Scheffold T, Uellner M, Schwarz F, Kubler W (1988) Non-invasive assessment of infarct reperfusion: The predictive power of the time to peak value of myoglobin, CKMB, and CK in serum. Eur Heart J 9:619–624
Apple FS (1992) Acute myocardial infarction and coronary reperfusion. Serum cardiac markers for the 1990s. Am J Clin Pathol 97:217–216
Laperche T, Steg PG, Benessiano J, Dehoux M, Juliard J-M, Himbert D, Gourgon R (1992) Pattern of myoglobin and MM creatine kinase isoform release early after intravenous thrombolysis or direct percutaneous transluminal coronary angioplasty for acute myocardial infarction and implications for the early noninvasive diagnosis of reperfusion. Am J Cardiol 70:1129–1134
O’Brien P, Shen H, McCutcheon L, O’Grady M, Byrne PJ, Ferguson HW, Mirsalimi MS, Julian RJ, Sargeant JM, Tremblay RRM, Blackwell TE (1992) Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: Use in various animals indicates functional role of myohemoproteins. Mol Cell Biochem 112:45–52
Van Nieuwenhoven FA, Kleine AH, Keizer HA, Van Dieijen-Visser MP, Van der Vusse GJ, Glate JFC (1992) Comparison of myoglobin and fatty acid-binding protein as plasma markers for muscle damage in man (abstract). Eur J Physiol 421:R40
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Yoshimoto, K., Tanaka, T., Somiya, K. et al. Human heart-type cytoplasmic fatty acid-binding protein as an indicator of acute myocardial infarction. Heart Vessels 10, 304–309 (1995). https://doi.org/10.1007/BF02911388
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DOI: https://doi.org/10.1007/BF02911388