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Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement

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Abstract

Objectives

Human heart-type fatty acid binding protein (HFABP) is a biomarker for diagnosis, risk assessment, and prognosis of acute myocardial infarction, and we aimed to establish an immunoassay for HFABP quantitation.

Methods

Human HFABP monoclonal antibodies (mAbs) were developed, evaluated by enzyme-linked immunosorbent assay, and a chemiluminescence enzyme immunoassay (CLEIA) generated. Analytical performance of the CLEIA was evaluated by measuring serum HFABP.

Results

The prokaryotically expressed rHFABP was purified and four anti-HFABP mAbs with superior detection performance were obtained after immunizing BALB/c mice. MAbs 2B8 and 6B3 were selected as respective capture and detection antibodies for HFABP measurement by CLEIA (detection range, 0.01–128 μg/L). Results using the CLEIA showed excellent correlation (r, 0.9622) and the correlation coefficient was 0.9809 (P < 0.05) by the Tukey test statistical analysis with those of latex-enhanced immunoturbidimetry in hospitals.

Conclusion

Our mAbs and CLEIA for HFABP detection represent new diagnostic tools for measurement of human serum HFABP.

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References

  • Colli A, Josa M, Pomar JL, Mestres CA, Gherli T (2007) Heart fatty acid binding protein in the diagnosis of myocardial infarction: where do we stand today? Cardiology 108(1):4–10

    Article  CAS  PubMed  Google Scholar 

  • Collinson P (2017) Laboratory medicine is faced with the evolution of medical practice. J Med Biochem 36(3):211–215

    Article  PubMed  PubMed Central  Google Scholar 

  • Da Molin S, Cappellini F, Falbo R, Signorini S, Brambilla P (2014) Verification of an immunoturbidimetric assay for heart-type fatty acid-binding protein (H-FABP) on a clinical chemistry platform and establishment of the upper reference limit. Clin Biochem 47(16–17):247–249

    Article  PubMed  Google Scholar 

  • Furuhashi M, Hotamisligil GS (2008) Fatty acid-binding proteins: Role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov 7:489–503

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Goel H, Melot J, Krinock MD, Kumar A, Nadar SK, Lip GYH (2020) Heart-type fatty acid-binding protein: an overlooked cardiac biomarker. Ann Med 52(8):444–461

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guan X, Liang T, Fan J, Yang W, Wu D, Li X (2022) The value of Copeptin, myocardial fatty acid-binding protein and myocardial markers in the early diagnosis of acute myocardial infarction. Am J Transl Res 14(11):8002–8008

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kabekkodu SP, Mananje SR, Saya RP (2016) A study on the role of heart type fatty acid binding protein in the diagnosis of acute myocardial infarction. J Clin Diagn Res 10(1): OC07–OC10

  • Kakoti A, Goswami P (2013) Heart type fatty acid binding protein: structure, function and biosensing applications for early detection of myocardial infarction. Biosens Bioelectron 43:400–411

    Article  CAS  PubMed  Google Scholar 

  • Kang K, Wu P, Li W, Tang S, Wang J, Luo X, Xie M (2015) Evaluation of a newly developed quantitative heart-type fatty acid binding protein assay based on fluorescence immunochromatography using specific monoclonal antibodies. Scand J Clin Lab Invest 75(8):693–698

    CAS  PubMed  Google Scholar 

  • Kim Y, Kim H, Kim SY, Lee HK, Kwon HJ, Kim YG, Lee J, Kim HM, So BH (2010) Automated heart-type fatty acid-binding protein assay for the early diagnosis of acute myocardial infarction. Am J Clin Pathol 134(1):157–162

    Article  CAS  PubMed  Google Scholar 

  • Liao M, Zheng J, Xu Y, Qiu Y, Xia C, Zhong Z, Liu L, Liu H, Liu R, Liang S (2021) Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum. J Immunol Methods 488:112913

    Article  CAS  PubMed  Google Scholar 

  • Lichtenauer M, Jirak P, Wernly B, Paar V et al (2017) A comparative analysis of novel cardiovascular biomarkers in patients with chronic heart failure. Eur J Intern Med 44:31–38

    Article  CAS  PubMed  Google Scholar 

  • Mihailescu CM, Stan D, Iosub R, Moldovan C, Savin M (2015) A sensitive capacitive immunosensor for direct detection of human heart fatty acid-binding protein (h-FABP). Talanta 132:37–43

    Article  CAS  PubMed  Google Scholar 

  • Otaki Y, Watanabe T, Kubota I (2017) Heart-type fatty acid-binding protein in cardiovascular disease: A systemic review. Clin Chim Acta 474:44–53

    Article  CAS  PubMed  Google Scholar 

  • Pyati AK, Devaranavadagi BB, Sajjannar SL, Nikam SV, Shannawaz M, Sudharani (2015) Heart-type fatty acid binding protein: a better cardiac biomarker than CK-MB and myoglobin in the early diagnosis of acute myocardial infarction. J Clin Diagn Res 9(10): BC08-BC11

  • Rezar R, Jirak P, Gschwandtner M, Derler R et al (2020) Heart-type fatty acid-binding protein (H-FABP) and its role as a biomarker in heart failure: what do we know so far? J Clin Med 9(1):164

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schernthaner C, Lichtenauer M, Wernly B, Paar V et al (2017) Multibiomarker analysis in patients with acute myocardial infarction. Eur J Clin Invest 47(9):638–648

    Article  CAS  PubMed  Google Scholar 

  • Shi Z, Xu Z, Hu J, Wei W et al (2022) Ascorbic acid-mediated organic photoelectrochemical transistor sensing strategy for highly sensitive detection of heart-type fatty acid binding protein. Biosens Bioelectron 201:113958

    Article  CAS  PubMed  Google Scholar 

  • Tarighi S, Najafi M, Hossein-Nezhad A, Ghaedi H, Meshkani R, Moradi N, Fadaei R, Kazerouni F, Shanaki M (2017) Association Between Two Common Polymorphisms of Vitamin D Binding Protein and the Risk of Coronary Artery Disease: A Case-control Study. J Med Biochem 36(4):349–357

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Topf A, Mirna M, Bacher N, Paar V et al (2021) Analysis of selected cardiovascular biomarkers in Takotsubo cardiomyopathy and the most frequent cardiomyopathies. Front Cardiovasc Med 8:700169

    Article  PubMed  PubMed Central  Google Scholar 

  • Van Hise CB, Greenslade JH, Parsonage W, Than M, Young J, Cullen L (2018) External validation of heart-type fatty acid binding protein, high-sensitivity cardiac troponin, and electrocardiography as rule-out for acute myocardial infarction. Clin Biochem 52:161–163

    Article  PubMed  Google Scholar 

  • Vera MA, Koch CD, Kavsak PA, El-Khoury JM (2021) Determination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population. Clin Chim Acta 523:397–401

    Article  CAS  PubMed  Google Scholar 

  • Watanabe T, Ohkubo Y, Matsuoka H, Kimura H et al (2001) Development of a simple whole blood panel test for detection of human heart-type fatty acid-binding protein. Clin Biochem 34(4):257–263

    Article  CAS  PubMed  Google Scholar 

  • Xu LQ, Yang YM, Tong H, Xu CF (2018) Early diagnostic performance of heart-type fatty acid binding protein in suspected acute myocardial infarction: evidence from a meta-analysis of contemporary studies. Heart Lung Circ 27(4):503–512

    Article  PubMed  Google Scholar 

  • Ye XD, He Y, Wang S, Wong GT, Irwin MG, Xia Z (2018) Heart-type fatty acid binding protein (H-FABP) as a biomarker for acute myocardial injury and long-term post-ischemic prognosis. Acta Pharmacol Sin 39(7):1155–1163

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zavala-Ortiz DA, Ebel B, Li MY, Barradas-Dermitz DM, Hayward-Jones PM, Aguilar-Uscanga MG, Marc A, Guedon E (2020) Interest of locally weighted regression to overcome nonlinear effects during in situ NIR monitoring of CHO cell culture parameters and antibody glycosylation. Biotechnol Prog 36(1):e2924

    Article  CAS  PubMed  Google Scholar 

  • Zavala-Ortiz DA, Denner A, Aguilar-Uscanga MG, Marc A, Ebel B, Guedon E (2022) Comparison of partial least square, artificial neural network, and support vector regressions for real-time monitoring of CHO cell culture processes using in situ near-infrared spectroscopy. Biotechnol Bioeng 119(2):535–549

    Article  CAS  PubMed  Google Scholar 

  • Zeng QH, Zhang XW, Xu XL, Jiang MH, Xu KP, Piao JH, Zhu L, Chen J, Jiang JG (2013) Antioxidant and anticomplement functions of flavonoids extracted from Penthorum chinense Pursh. Food Funct 4(12):1811–1818

    Article  CAS  PubMed  Google Scholar 

  • Zhu L, Fu W, Zhu B, Feng Q et al (2023) An integrated microfluidic electrochemiluminescence device for point-of-care testing of acute myocardial infarction. Talanta 262:124626

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by National Natural Science Foundation of China (No. 81702005), Hunan Provincial Natural Science Foundation of China (No. 2019JJ50370), Science and Technology Plan Project of Changsha (No. KQ1801039 and KQ1907130), Science and Technology Plan of Hunan Provincial People's Hospital (No. RS20160208), Scientific Research Project of Hunan Provincial Health Commission (B202311009257).

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Author notes

  1. Jiao Zheng and Yilan Qiu make equal contribution to this paper.

Authors and Affiliations

  1. School of Medicine, Hunan Normal University, Changsha, 410013, China

    Jiao Zheng, Ye Xu, Meifang Quan, Zhihong Zhong, Qinglin Wang, Xuan Zeng & Rushi Liu

  2. Immunodiagnostic Reagents Engineering Research Center of Hunan Province, Hunan Normal University, Changsha, 410013, China

    Jiao Zheng, Yilan Qiu, Ye Xu, Meifang Quan, Zhihong Zhong, Qinglin Wang, Yi Wu, Xuan Zeng & Rushi Liu

  3. The First Affiliated Hospital of Hunan Normal University, Changsha, 410008, China

    Yi Wu

  4. College of Life Science, Hunan Normal University, Changsha, 410013, China

    Yilan Qiu

  5. Department of Laboratory Medicine, The First People’s Hospital of Chenzhou, Chenzhou, 423000, China

    Chuan Xia

Authors
  1. Jiao Zheng
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  2. Yilan Qiu
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  8. Xuan Zeng
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  9. Chuan Xia
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  10. Rushi Liu
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Contributions

RSL, JZ and YLQ designed the study. JZ, ZHZ, YW, QLW and RSL drafted and revised the manuscript. JZ, YX, XZ, and MFQ performed the research, collected data and analyzed the data. CX revised the manuscript. All the authors reviewed and approved the final manuscript.

Corresponding author

Correspondence to Rushi Liu.

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Zheng, J., Qiu, Y., Xu, Y. et al. Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement. Biotechnol Lett 45, 1431–1440 (2023). https://doi.org/10.1007/s10529-023-03425-4

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