Skip to main content
SpringerLink
Log in

Abundant dendritic cells express HLA-DR in pleomorphic adenomas

  • Published:
Virchows Archiv B

Summary

Most pleomorphic adenomas were found to contain abundant dendritic cells (DC) with major histo-compatibility complex (MHC) class II (HLA-DR) expression. Their immunohistochemical staining features were suggestive of dendritic histiocytic cells. Extensive phenotypic characterization by two-colour immunofluorescence staining for various cell markers was performed. The DC expressed both HLA class I and II determinants, vimentin, S-100 protein, and various monocyte-related markers (10G11, 3D10, 7G5 or CD11a, 8C2) but were negative for leucocyte common antigen (CD45), Leu-6 (CD1), and the myelomonocytic L1 antigen. Characterization of HLA-DR positive DC isolated by an immunomagnetic bead method confirmed the immunohistochemical staining pattern that corresponds to the phenotype of interdigitating cells. Morphological and immunological implications of the abundant presence of these cells in pleomorphic adenomas are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adamson TC, Fox RI, Frisman DM, Howell FV (1983) Immunohistological analysis of lymphoid infiltrates in primary Sjögren’s syndrome using monoclonal antibodies. J Immunol 130:203–208

    PubMed  Google Scholar 

  • Brandtzaeg P (1974) Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with cold ethanol-fixation technique. Immunology 26:1101–1114

    CAS  Google Scholar 

  • Brandtzaeg P, Rognum TO (1983) Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocytochemistry of lymphomas. Histochem J 15:655–689

    Article  PubMed  CAS  Google Scholar 

  • Brandtzaeg P, Dale I, Fagerhol MK (1987) Distribution of a formalin-resistant myelomonocytic antigen (L1) in human tissues. I. Comparison with other leukocyte markers by paired immunofluorescence and immunoenzyme staining. Am J Clin Pathol 87:681–699

    PubMed  CAS  Google Scholar 

  • Dale I, Brandtzaeg P, Fagerhol MK, Scott H (1985) Distribution of a new myelomonocytic antigen (L1) in human peripheral blood leukocytes. Immunofluorescence and immunoperoxidase staining features in comparison with lysozyme and lactoferrin. Am J Clin Pathol 84:24–34

    PubMed  CAS  Google Scholar 

  • David R, Buchner A (1980) Langerhans’ cells in a pleomorphic adenoma of submandibular salivary gland. J Pathol 131:127–135

    Article  PubMed  CAS  Google Scholar 

  • De Waal RMW, Semeijn JT, Cornelissen IMH, Ramaekers FCS (1984) Epidermal Langerhans cells contain intermediate-sized filaments of the vimentin type: an immunocytologic study. J Invest Dermatol 82:602–604

    Article  PubMed  Google Scholar 

  • Delsol G, Al Saati T, Caveriviere P, Voigt JJ, Ancelin E, Rigal-Huguet F (1984) Immunoperoxidase study of normal and pathological lymphoid tissues: Advantages of monoclonal antibodies. Ann Pathol 4:165–183

    PubMed  CAS  Google Scholar 

  • Fithian E, Kung P, Goldstein G, Rubenfeld M, Fenoglio C, Edelson R (1981) Reactivity of Langerhans cells with hybridoma monoclonal antibody. Proc Natl Acad Sci USA 78:2541–2544

    Article  PubMed  CAS  Google Scholar 

  • Fox RI, Bumol T, Fantozzi R, Bone R (1986) Expression of histocompatibility antigen HLA-DR by salivary gland epithelial cells in Sjögren’s syndrome. Arthritis Rheum 29:1105–1111

    Article  PubMed  CAS  Google Scholar 

  • Hammar S, Bockus D, Remington F, Bartha M (1986) The wide-spread distribution of Langerhans cells in pathologic tissues: an ultrastructural and immunohistochemical study. Hum Pathol 17:894–905

    PubMed  CAS  Google Scholar 

  • Hoffman-Fezer G, Gotze D, Rodt H, Thierfelder S (1978) Immunohistochemical localization of xenogenic antibodies against Ia lymphocytes on B cells and reticular cells. Immunogenetics 6:367–377

    Article  Google Scholar 

  • Hsu S-M, Crossman J, Jaffe ES (1983) Lymphocyte subsets in normal human lymphoid tissues. Am J Clin Pathol 80:21–30

    PubMed  CAS  Google Scholar 

  • Huitfeldt HS, Brandtzaeg P (1985) Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium. Histochemistry 83:381–389

    Article  PubMed  CAS  Google Scholar 

  • Inaba K, Schuler G, Witmer MD, Valinsky J, Atassi B, Steinman RM (1986) Immunologic properties of purified epidermal Langerhans cells. Distinct requirements for stimulation of unprimed and sensitized T lymphocytes. J Exp Med 164:605–613

    Article  PubMed  CAS  Google Scholar 

  • Knight SC (1984) Veiled cells “dendritic cells” of the peripheral lymph. Immunobiology 168:349–361

    PubMed  CAS  Google Scholar 

  • Knight S, Hunt R, Dore C, Medawar PB (1985) Influence of dendritic cells on tumor growth. Proc Natl Acad Sci USA 82:4495–4497

    Article  PubMed  CAS  Google Scholar 

  • Kraal G, Breel M, Janse M, Bruin G (1986) Langerhans’ cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med 163:981–997

    Article  PubMed  CAS  Google Scholar 

  • Lindahl G, Hedfors E, Klareskog L, Forsum U (1985) Epithelial HLA-DR expression and T lymphocyte subsets in salivary glands in Sjögren’s syndrome. Clin Exp Immunol 61:475–482

    PubMed  CAS  Google Scholar 

  • Mason DY (1985) Immunohistochemical labeling of monoclonal antibodies by the APAAP immunoalkaline phosphate technique. In: Bullock GR, Petrusz P (eds) Immunocytochemistry, vol 3. Academic Press, London, pp 25–42

    Google Scholar 

  • Matthews JB, Potts AJC, Hamburger J, Scott DGI (1985) T6 antigen-positive cells in human labial salivary glands. Archs Oral Biol 30:325–329

    Article  CAS  Google Scholar 

  • McMillan EM, Brubaker DB, Peters S et al. (1983) Demonstration of cells bearing the OKT6 determinant in human tonsil and lymph node. Cancer Immunol Immunother 15:221–226

    Article  PubMed  CAS  Google Scholar 

  • Mierau GW, Favara BE (1986) S-100 protein immunohistochemistry and electron microscopy in the diagnosis of Langerhans cell proliferative disorders: a comparative assessment. Ultrastruct Pathol 10:303–309

    Article  PubMed  CAS  Google Scholar 

  • Moll R, Franke WW, Schiller DL, Geiger B, Krepier R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Article  PubMed  CAS  Google Scholar 

  • Mukai K, Rosai J, Burgdorf WHC (1980) Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol 4:273–276

    Article  PubMed  CAS  Google Scholar 

  • Murphy GF, Bhau AK, Sato S, Mihm MC, Harrist TJ (1981) A new immunologie marker for human Langerhans cells. N Engl J Med 304:781–792

    Google Scholar 

  • Oxholm PO, Manthorpe R, Oxholm A, Schiödt M (1985) Brief report. Langerhans cells in labial minor salivary glands in primary Sjögren’s syndrome. Acta Pathol Microbiol Immunol Scand Sect A 93:105–107

    CAS  Google Scholar 

  • Poppema S, Bhan AK, Reinherz EL, McClusky RT, Schlossman SF (1981) Distribution of T cell subsets in human lymph nodes. J Exp Med 153:30–41

    Article  PubMed  CAS  Google Scholar 

  • Raphael M, Debre P, Charron DJ (1984) Reduced Ia-positive Langerhans’ cells in AIDS (letter). N Engl J Med 311:858

    Google Scholar 

  • Reinherz EL, Rubenfeld M, Fenoglio C, Edelson R, Kung PC, Goldstein G, Levey RH, Schlossman SF (1980) Discrete stages of human intrathymic differentiation: Analysis of normal thymocyte and leukaemic lymphoblasts of T cell lineage. Proc Natl Acad Sci USA 77:1588–1592

    Article  PubMed  CAS  Google Scholar 

  • Roop DP, Cheng CK, Toftgard R, Stanley JR, Steinert PM, Yuspa SH (1985) The use of cDNA clones and monospecific antibodies as probes to monitor keratin gene expression. Ann NY Acad Sci 455:426–435

    Article  PubMed  CAS  Google Scholar 

  • Schuler G, Steinman RM (1985) Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J Exp Med 161:526–546

    Article  PubMed  CAS  Google Scholar 

  • Schuler G, Romani N, Steinman RM (1985) A comparison of murine epidermal Langerhans cells with spleen dendritic cells. J Invest Dermatol 85:99s-106s

    Article  PubMed  CAS  Google Scholar 

  • Segerberg-Konttinen M, Bergroth V, Jungeil P, Malmstrom M, Nordström D, Sane J, Immonen I, Konttinen YT (1987) T lymphocyte activation state in the minor salivary glands of patients with Sjögren’s syndrome. Annals Rheum Dis 46:649–653

    Article  CAS  Google Scholar 

  • Skoskiewicz MJ, Colvin RB, Schneeberger EE, Russel PS (1985) Widespread and selective induction of major histocompatibility complex-determined antigens in vivo by gamma interferon. J Exp Med 162:1645–1664

    Article  PubMed  CAS  Google Scholar 

  • Steinman RM, Nussenzweig MC (1980) Dendritic cells: features and functions. Immunol Rev 53:127–147

    Article  PubMed  CAS  Google Scholar 

  • Takahashi K, Isobe T, Ohtsuki Y, Sonobe H, Takeda I, Akagi T (1984) Immunohistochemical localization and distribution of S-100 proteins in human lymphoreticular system. Am J Pathol 116:497–503

    PubMed  CAS  Google Scholar 

  • Thrane PS, Roop DR, Sollid LM, Huitfeldt HS, Brandtzaeg P (1990) Two-colour immunofluorescence marker study of pleomorphic adenomas. Histochemistry 93:459–468

    Article  PubMed  CAS  Google Scholar 

  • Thrane PS, Rognum TO, Huitfeldt HS, Brandtzaeg P (1987) The distribution of HLA-DR determinants, secretory component (SC), amylase, lysozyme, IgA, IgM, and IgD in fetal and postnatal salivary glands. Adv Exp Med Biol 216B: 1431–1438

    PubMed  CAS  Google Scholar 

  • Vaines K, Brandtzaeg P (1985) Retardation of immunfluorescence fading during microscopy. J Histochem Cytochem 83:381–389

    Google Scholar 

  • Van der Oord JJ, De Wolf-Peeters C, Desmet VJ, Takahashi K, Ohtsuki Y, Akagi T (1985) Nodular alteration of the paracortical area. An in situ immunohistochemical analysis of primary, secondary, and tertiary T-nodules. Am J Pathol 120:55–66

    PubMed  Google Scholar 

  • Vartdal F, Gaudernack G, Funderud S, Bratlie A, Lea T, Ugelstad J, Thorsby E (1986) HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation-a fast and reliable technique. Tissue Antigens 28:301–312

    PubMed  CAS  Google Scholar 

  • Watamabe S, Nakajima T, Shimosato Y, Shimamura K, Sakuma H (1983) T-zone histiocytes with S100 protein. Development and distribution in human fetuses. Acta Pathol Jpn 33:15–22

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, Medical Faculty, The National Hospital, Rikshospitalet, Norway

    Per Stanley Thrane, Ludvig Magne Sollid & Per Brandtzaeg

  2. Department of Oral Surgery and Oral Medicine, Dental Faculty, University of Oslo, Oslo, Norway

    Per Stanley Thrane

Authors
  1. Per Stanley Thrane
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ludvig Magne Sollid
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Per Brandtzaeg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thrane, P.S., Sollid, L.M. & Brandtzaeg, P. Abundant dendritic cells express HLA-DR in pleomorphic adenomas. Virchows Archiv B Cell Pathol 59, 195–203 (1990). https://doi.org/10.1007/BF02899405

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02899405

Key words

Search

Navigation