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Two-colour immunofluorescence marker study of pleomorphic adenomas

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Summary

Extensive use of two-colour immunofluorescence staining for various cell markers in pleomorphic adenoma, revealed three consistent phenotypic features: (1) keratin polypeptide No. 14, which was virtually restricted to myoepithelial cells (MEC) in normal salivary glands, appeared in a large fraction of the tumour cells, suggesting that the principal neoplastic element is derived from MEC or their immediate precursors; (2) a complex co-expression pattern of various cell markers was found, with extensive concurrence of keratin and vimentin in strands of MEC-like and myxoid tumour cells, probably reflecting different degrees of tumour cell differentiation; and (3) two phenotypically distinctive dendritic cell populations were identified, one consisting of keratin positive tumour cells and the other of HLA-DR positive but keratin negative stromal cells. The significance of these findings with regard to the histogenesis and complex morphology of pleomorphic adenoma is discussed.

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Authors and Affiliations

  1. Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, Medical Faculty, The National Hospital, Rikshospitalet, Norway

    P. S. Thrane, L. M. Sollid, H. S. Huitfeldt & P. Brandtzaeg

  2. Department of Oral Surgery and Oral Medicine, Dental Faculty, University of Oslo, Oslo, Norway

    P. S. Thrane

  3. Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland, USA

    D. R. Roop

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  1. P. S. Thrane
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  2. D. R. Roop
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  3. L. M. Sollid
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  4. H. S. Huitfeldt
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  5. P. Brandtzaeg
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Thrane, P.S., Roop, D.R., Sollid, L.M. et al. Two-colour immunofluorescence marker study of pleomorphic adenomas. Histochemistry 93, 459–468 (1990). https://doi.org/10.1007/BF00266401

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