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Immunohistochemical localization of xenogeneic antibodies against Iak lymphocytes on B cells and reticular cells

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Abstract

Rabbit antiserum against B10.AQR mouse spleen and lymph node cells (RAQR), after appropriate absorption, reacted with Iak-positive spleen and lymph node cells in cytotoxic and complement-fixing indicator systems. It reacted neither with Iak-positive thymocytes nor Iak-negative thymocytes, spleen, and lymph node cells. Cryostat sections of tissue from Iak-positive and Iak-negative mice were incubated with RAQR and either rabbit anti-mouse Ig or rabbit anti-T cell globulin. With the unlabeled antibody enzyme method, RAQR-stained lymphocytes were concentrated in the B-cell regions of spleen and lymph nodes of Iak-positive CBA mice. The tissues of mice bearingI-region haplotypes different fromk were negative. Reticular cells of the T cell-supporting network were also positive in Iak mice, but liver, gall bladder, and testicular cells were not. Macrophages of both Iak-positive and -negative mice were stained by RAQR and also by heat-aggregated, peroxidase-labeled Ig. Iak-positive reticular cells survived 900 R total body irradiation and persisted after grafting with Iak-negative bone marrow. The reticular cells were also seen in a thymus which was depleted of cortisone-sensitive lymphocytes.

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Abbreviations

RAQR:

rabbit anti-mouse-B10.AQR globulin

RAMTG:

rabbit anti-mouse T-cell globulin

RAMIG:

rabbit anti-mouse Ig

SARIG:

sheep anti-rabbit Ig

agg-HIg:

aggregated human Ig

PAP:

anti-peroxidase-peroxidase-complex

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Author notes

  1. Dietrich Götze

    Present address: The Wistar Institute, Philadelphia, Pennsylvania

Authors and Affiliations

  1. Institut für Hämatologie, Landwehrstraße 61, Munich, Germany

    Gertrud Hoffmann-Fezer, Hans Rodt & Stefan Thierfelder

Authors
  1. Gertrud Hoffmann-Fezer
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  2. Dietrich Götze
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  3. Hans Rodt
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  4. Stefan Thierfelder
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Hoffmann-Fezer, G., Götze, D., Rodt, H. et al. Immunohistochemical localization of xenogeneic antibodies against Iak lymphocytes on B cells and reticular cells. Immunogenetics 6, 367–377 (1978). https://doi.org/10.1007/BF01563928

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  • DOI: https://doi.org/10.1007/BF01563928

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