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Immunohistochemical and biochemical detection of uridine-diphosphate-glucuronyltransferase (UDP-GT) activity in putative preneoplastic liver foci

  • Published:
Virchows Archiv B Cell Pathology Including Molecular Pathology

Summary

Preneoplastic liver foci were produced in female Wistar rats by the administration of 2-acetylaminofluorene (0.03% w/w) in the diet for 174 days. Increased UDP-glucuronyltransferase (UDP-GT) could be visualized immunohistochemically in the same focal areas which were ATPase-negative andγ-glutamyltranspeptidase-positive. Immunohistochemical detection was possible using rabbit anti-UDP-GT and peroxi-dase-labeled swine anti-rabbit immunoglobulins. The results of immuno-histochemistry were substantiated by enzyme determination in micro-dissected material. UDP-GT activity was 5-fold higher in focal areas in comparison with the surrounding liver tissue.

Increased UDP-GT activity in conjunction with the altered pattern of other drug-metabolizing enzymes is consistent with increased resistance of preneoplastic cells to the cytotoxicity of carcinogens. Immunohistochemical detection of UDP-GT may provide a new marker for preneoplastic lesions which, in conjunction with other markers, may prove useful in analyzing the various stages of liver carcinogenesis and the remodeling of preneoplastic lesions after cessation of carcinogenic stimuli.

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Authors and Affiliations

  1. Zentrum Pathologie, Universität Göttingen, Robert-Koch-Str. 40, D-3400, Göttingen

    G. Fischer & A. Schauer

  2. Zentrum Pharmakologie, Universität Göttingen, Kreuzbergring 57, D-3400, Göttingen

    D. Ullrich & K. W. Bock

  3. Zentrum Biochemie, Universität Göttingen, Humboldtallee 7, D-3400, Göttingen, Federal Republic of Germany

    N. Katz

Authors
  1. G. Fischer
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  2. D. Ullrich
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  3. N. Katz
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  4. K. W. Bock
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  5. A. Schauer
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Supported by the Bundesministerium für Forschung und Technology, UC-Teilprojekt CMT

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Fischer, G., Ullrich, D., Katz, N. et al. Immunohistochemical and biochemical detection of uridine-diphosphate-glucuronyltransferase (UDP-GT) activity in putative preneoplastic liver foci. Virchows Archiv B Cell Pathol 42, 193–200 (1983). https://doi.org/10.1007/BF02890382

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  • DOI: https://doi.org/10.1007/BF02890382

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