Abstract
The influence of increasing concentrations of copper, zinc, lead, nickel, chromium and cadmium on 14-day-old seedlings of wheat (Triticum aestivum L. cv. Vergina) was studied. Plants were grown in 1/10 strength Rorison’s nutrient solution with increasing concentrations of each of the metals added separately. The toxicity of metals depressed shoot growth but the most evident symptoms were on roots. The concentration of each metal which caused inhibition of root growth was chosen to study the influence of metals on isoperoxidases of wheat shoots. The concentrations employed did not alter the number of peroxidase bands but almost in all cases enhanced the intensities of bands of pH 4.0-4.2 and 5.0-5.4, while they decreased the intensities of bands of pH 4.2-4.6 and 5.4-6.5.
The similar effects of the different heavy metals employed may suggest similarity in metal action on wheat isoperoxidases. The increased intensities of peroxidase bands may be considered as an indication of enhanced senescence caused by the heavy metal treatments.
Generally, our results suggest that the heavy metals employed have caused complex changes on the multiple forms of peroxidases.
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References
Agarwala, S. C., Kumar, A., Sharma, C. P.: Effect of excess supply of heavy metals on barley during germination, with special reference to catalase and peroxidase. - Nature191: 726–727,1961.
Antonovics, J., Bradshaw, A. D., Turner, R.: Heavy metal tolerance in plants. - Adv. ecol. Res.7:1–85, 1971.
Braber, J. M.: Catalase and peroxidase in primary bean leaves during development and senescence. - Z. Pflanzenphysiol.97:135–144,1980.
Eleftheriou, E. P., Karataglis, S.: Ultrastructural and morphological characteristics of cultivated wheat growing on copper-polluted fields. - Bot. Acta102: 134–140,1989.
Kar, M., Feierabend, J.: Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence. - Planta160: 385–391, 1984.
Karataglis, S.: Estimation of the toxicity of different metals, using as criterion the degree of root elongation inTriticum aestivum seedlings. - Phyton (Austria)26: 209–217, 1987.
Lee, K. G., Gunningham, B. A., Paulsen, G. M., Liang, G. H., Moore, R. B.: Effects of cadmium on respiration rate and activities of several enzymes in soybean seedlings. - Physiol. Plant.36:4–6,1976.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem.193: 265–275,1951.
Maier, R.: Aktivität und multiple Formen der Peroxydase in unverbleiten und verbleiten Pflanzen vonZea mays undMedicago sativa. - Phyton (Austria)19: 83–96, 1978.
Moustakas, M., Coucoli, E., Tsekos, I.: Isoelectric focusing of seed proteins from natural greek populations of twoAgropyron species. - In : Stathakos, D. (ed.) : Electrophoresis ’82. Pp. 725–731. Walter de Gruyter and Co., Berlin 1983.
Mukherji, S., Das Gupta, B.: Characterization of copper toxicity in lettuce seedlings. - Physiol. Plant.27: 126–129, 1972.
Symeonidis, L., Karataglis, S., Tsekos, I.: Electrophoretic variation in esterases and peroxisases of native greek diploidAegilops species (Ae. caudata andAe. comosa, Poaceae). - Plant. Syst. Evol.131 : 1–15, 1979.
Symeonidis, L., McNeilly, T., Bradshaw, A. D.: Differential tolerance of three cultivare of Agrostiscapillaris L. to cadmium, copper, lead, nickel and zinc. - New Phytol.101: 309–315, 1985.
Thomas, H., Stoddart, J. L.: Leaf senescence. - Annu. Rev. Plant Physiol.31: 83–111, 1980.
Upadhyaya, A., Sankhla, D., Davis, T. D., Sankhla, N., Smith, B. N.: Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. - J. Plant Physiol.121: 453–461, 1985.
Van Assche, F., Clijsters, H.: Enzyme analysis in plants as a tool for assessing phytotoxicity of heavy metal polluted soils. -Med. Fac. Landbouww. Rijksuniv. Gent52: 1819–1824, 1987.
Van Assche, F., Put, C., Clijsters, H.: Heavy metals induce specific isozyme patterns of peroxidase inPhaseolus vulgaris L. - Arch. int. Physiol. Biochim.94: 60, 1986,
Wainwright, S. J., Woolhouse, H. W.: Physiological mechanisms of heavy metal tolerance in plants. - In : The Ecology of Resource Degradation and Renewal. 15th Symposium of the British Ecological Society. Pp. 231–257. Blackwell Sci. Publ. Oxford 1975.
Weigel, H. J., Jäger, H. J.: Different effects of cadmiumin vitro andin vivo on enzyme activities in bean plants(Phaseolus vulgaris L. cv. Sankt Andreas). - Z. Pflanzenphysiol.97: 103–113, 1980.
Wong, M., Bradshaw, A. D.: A comparison of the toxicity of heavy metals, using root elongation of rye grass,Lolium perenne. - New Phytol.91: 255–261, 1982.
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Karataglis, S., Moustakas, M. & Symeonidis, L. Effect of heavy metals on isoperoxidases of Wheat. Biol Plant 33, 3–9 (1991). https://doi.org/10.1007/BF02873778
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DOI: https://doi.org/10.1007/BF02873778