Summary
Somatic embryos which originated from mature embryo axes of the chickpea (Cicer arietinum L.) showed varied morphologies. Embryos were classified based on shape of the embryo and number of cotyledons. “Normal” (zygotic-like) embryos were bipolar structures with two cotyledons and a well-developed shoot and root apical meristem, whereas “aberrant” embryos were horn-shaped, had single and multiple cotyledons, and were fasciated. Histological examination revealed the absence of a shoot apical meristem in horn-shaped embryos. Fasciated embryos showed diaxial fusion of two embryos. Secondary embryogenesis was also observed, in which the embryos emerged from the hypocotyl and cotyledonary region of the primary somatic embryo. This report documents the absence of an apical meristem as a vital factor in the lack of conversion of aberrant somatic embryos.
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Suhasini, K., Sagare, A.P. & Krishnamurthy, K.V. Study of aberrant morphologies and lack of conversion of somatic embryos of chickpea (Cicer arietinum L.). In Vitro Cell Dev Biol – Plant 32, 6–10 (1996). https://doi.org/10.1007/BF02823005
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DOI: https://doi.org/10.1007/BF02823005