Abstract
Peroxynitrite was tested for its effects on the opportunistic pathogenic fungusAspergillus fumigatus. It did not kill any dormant or swollen (4 h in a glucose-peptone medium) conidia in concentrations up to 6.25 mmol/L and the growth of germlings (after 6 or 9 h) was only slightly inhibited by 5 mmol/L peroxynitrite. The peroxynitrite donor SIN-1 (up to 10 mmol/L, I d in buffer) did not kill any conidia but inhibited their germination and growth, depending on the medium. Ten mmol/L SIN-1 in a poor medium was fungistatic and germination was stopped for 20 h. Nine strains ofA. fumigatus showed resistance comparable to the model strain, while 6Candida albicans strains were much more susceptible to both peroxynitrite and its donor. The results indicate that peroxynitrite does not contribute substantially to the antifungal activity of phagocytes againstA. fumigatus.
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Assreuy J., Cunha F.Q., Epperlein M., Noronha-Dutra A., O'Donnell C.A., Liew F.Y., Moncada S.: Production of nitric oxide and superoxide by activated macrophages and killing ofLeishmania major.Eur. J. Immunol. 24, 672–676 (1994).
Bartosz G.: Peroxynitrite: mediator of the toxic action of nitric oxide.Acta Biochim. Polon. 43, 645–659 (1996).
Bocca A.L., Hayashi E.E., Pinheiro A.G., Furlanetto A.B., Campanelli A.P., Cunha F.Q., Figuereido F.: Treatment ofParacoccidioides brasiliensis-infected mice with a nitric oxide inhibitor prevents the failure of cell-mediated immune response.J. Immunol. 161, 3056–3063 (1998).
Bogdan C.: Of microbes, macrophages and nitric oxide.Behring Inst. Mitt. 99, 58–72 (1997).
Brunelli L., Crow J.P., Beckman J.S.: The comparative toxicity of nitric oxide and peroxynitrite toEscherichia coli.Arch. Biochem. Biophys. 316, 327–334 (1995).
Cogliati M., Roverselli A., Boelaert J.R., Taramelli D., Lombardi L., Viviani M.A.: Development of anin vitro macrophage system to assessPenicillium marneffei growth and susceptibility to nitric oxide.Infect. Immun. 65, 279–284 (1997).
Ducrocq C., Blanchard B., Pignatelli B., Ohshima H.: Peroxynitrite: an endogenous oxidizing and nitrating agent.Cell. Mol. Life Sci. 55, 1068–1077 (1999).
Fernandes P.D., Assreuy J.: Role of nitric oxide and superoxide inGiardia lamblia killing.Braz. J. Med. Biol. Res. 30, 93–99 (1997).
Fierro I.M., Barja-Fidalgo C., Cuñha F.Q., Ferreira S.H.: The involvement of nitric oxide in the anti-Candida albicans activity of rat neutrophils.Immunology 89, 295–300 (1996).
Gross N.T., Nessa K., Camner D., Jarstrand C.: Production of nitric oxide by rat alveolar macrophages stimulated byCryoptococcus neoformans orAspergillus fumigatus.Med. Mycol. 37, 151–157 (1999).
Houk K.N., Condroski K.R., Pryor W.A.: Radical and concerted mechanisms in oxidations of amines, sulfides, and alkenes by peroxynitrite, peroxynitrous acid, and the peroxynitrite-CO2 adduct.J. Amer. Chem. Soc. 118, 13002–13006 (1996).
Koutný M.: From NO-confidence to nitric oxide acknowledgement: a story of bacterial nitric-oxide reductase.Folia Microbiol. 45, 197–204 (2000).
Kudeken N., Kawakami K., Saito A.: Different susceptibilities of yeasts and conidia ofPenicillium marneffei to nitric oxide (NO)-mediated fungicidal activity of murine macrophages.Clin. Exp. Immunol. 112, 187–193 (1998).
Kunert J.: Effect of nitric oxide donors on survival of conidia, germination and growth ofAspergillus fumigatus in vitro.Folia Microbiol. 40, 238–244 (1995).
Latgé J.P.:Aspergillus fumigatus and aspergillosis.Clin. Microbiol. Rev. 12, 310–350 (1999).
MacMicking J., Xie Q.W., Nathan C.: Nitric oxide and macrophage function.Ann. Rev. Immunol. 15, 323–350 (1997).
Michaliszyn E., Sénéchal S., Martel P., de Repentigny L.: Lack of involvement of nitric oxide in killing ofAspergillus fumigatus conidia by pulmonaryalveolar macrophages.Infect. Immun. 63, 2075–2078 (1995).
Pryor W.A., Squadrito G.L.: The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide.Amer. J. Physiol. 12, L699-L722 (1995).
Pryor W.A., Lemercier J.N., Zhang H.W., Uppu R.M., Squadrito G.L.: The catalytic role of carbon dioxide in the decomposition of peroxynitrite.Free Radical Biol. Med. 23, 331–338 (1997).
Romani L., Howard D.H.: Mechanisms of resistance to fungal infections.Curr. Opin. Immunol. 7, 517–523 (1995).
Sigler K., Chaloupka J., Brozmanová J., Stadler N., Höfer M.: Oxidative stress in microorganisms—I. Microbialvs. higher cells—damage and defenses in relation to cell aging and death.Folia Microbiol. 44, 587–624 (1999).
Squadrito G.L., Pryor W.A.: The nature of reactive species in systems that produce peroxynitrite.Chem. Res. Toxicol. 11, 718–719 (1998).
Taramelli D., Malabarba M.G., Sala G., Basilico N., Cocuzza G.: Production of cytokines by alveolar and peritoneal macrophages stimulated byAspergillus fumigatus conidia or hyphae.J. Med. Vet. Mycol. 34, 49–56 (1996).
Vázquez-Torres A., Jones-Carson J., Balish E.: Nitric oxide production does not directly increase macrophage candidacidal activity.Infect. Immun. 63, 1142–1144 (1995).
Vázquez-Torres A., Jones-Carson J., Balish E.: Peroxynitrite contributes to the candidacidal activity of nitric oxide-producing macrophages.Infect. Immun. 64, 3127–3133 (1996).
Vázquez-Torres A., Balish E.: Macrophages in resistance to candidiasis.Microbiol. Mol. Rev. 61, 170–192 (1997).
Xie Q., Kawakami K., Kudeken N., Zhang T., Qureshi M.H., Saito A.: Different susceptibility of three clinically isolated strains ofCryptococcus neoformans to the fungicidal effects of reactive nitrogen and oxygen intermediates: possible relationships with virulence.Microbiol. Immunol. (Japan) 41, 725–731 (1997).
Zou M.H., Ullrich V.: Peroxynitrite formed by simultaneous generation of nitric oxide and superoxide selectively inhibits bovine aortic prostacyclin synthase.FEBS Lett. 3382, 101–104 (1996).
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Kunert, J. Effect of Peroxynitrite on dormant spores and germlings ofAspergillus fumigatus in vitro . Folia Microbiol 45, 325–329 (2000). https://doi.org/10.1007/BF02817555
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DOI: https://doi.org/10.1007/BF02817555