Abstract
A new screening method for simultaneous detection of endo-β-1,4-mannanase and endo-β-1,4-xylanase producing microorganisms is described. Two differently dyed substrate Ostazin Brilliant Red-galactomannan and Remazol Brilliant Blue-xylan were incorporated into the same agar media. Decolorizing of one or both substrates around the cell colonies indicates secretion of the corresponding enzyme(s). The method was used to screen 449 yeasts and yeast-like microorganisms belonging to 68 different genera. The secretion of endo-β-1,4-mannanases and/or endo-β-1,4-xylanases was found within 10 genera (42 positive strains out of 261 tested). A low frequency of occurrence of endo-β-1,4-mannanases was observed within the generaCryptococcus (1 positive strain out of 15 tested),Geotrichum (1 of 6) andPichia (1 of 35). The highest frequency of occurrence of endo-β-1,4-mannanases was found within the generaStephanoascus (2 of 2) andAureobasidium (14 of 14). Strains hydrolyzing Ostazin Brilliant Red-galactomannan were cultivated in liquid media containing 1 % locust bean gum. The best producers of extracellular endo-β-1,4-mannanases were found to be the strains ofAureobasidium pullulans.
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References
Aspinall G.O.: Mannans, galactomannans and glucomannans, pp. 85–93 inPolysaccharides. Pergamon Press, Oxford 1970.
Berndt H., Liese W.: Untersuchungen über die Enzyme von Bläuepilzen. III. Pectintranseliminase und Mannanase beiAureobasidium pullulans.Arch. Mikrobiol. 79, 140–146 (1971).
Biely P., Krátky Z., Kocková-Kratochvílová A., Bauer Š.: Xylan degrading activity in yeasts: growth on xylose, xylan and hemicelluloses.Folia Microbiol. 23, 366–371 (1978).
Biely P., Mislovičová D., Toman R.: Soluble chromogenic substrates for the assay of endo-β-1,4-xylanases and endo-β-1,4-glycanases.Anal. Biochem. 144, 142–146 (1985).
Buchert J., Salminen J., Siika-Aho M., Ranua M., Vikari L.: The role ofTrichoderma reesei xylanase and mannanase in the treatment of softwood kraft pulp prior to bleaching.Holzforschung 49, 473–478 (1993).
Clark T.A., McDonald A.G., Senior D.J., Mayers P.R.: Mannanase and xylanase treatments of softwood chemical pulps: effects on pulp properties and bleachability, pp. 153–167 in K. Kirk, H.H.M. Chang (Eds):Biotechnology in Pulp and Paper Manufacture. Butterworth & Heinemann, Boston 1990.
Coughlan M.P., Hazlewood G.P.:Hemicellulose and Hemicellulases. Portland Press, London 1993.
Dekker R.F.H.: Biodegradation of the hemicelluloses, pp. 505–533 inBiosynthesis and Biodegradation of Wood Components (T. Higuchi, Ed.). Academic Press, Orlando (Florida) 1985.
Dekker R.F.H., Richards G.N.: Hemicellulases: their occurrence, purification, properties and mode of action.Adv. Carbohydr. Chem. Biochem. 32, 277–352 (1976).
Deshpande M.S., Rale V.B., Lynch J.M.:Aureobasidium pullulans in applied microbiology: A status report.Enzyme Microb. Technol. 14, 514–527 (1992).
Eriksson K.-E.L., Blanchette R.A., Ander P.:Microbial and Enzymatic Degradation of Wood and Wood Components. Springer-Verlag, Berlin 1990.
Farkaš V., Líšková M., Biely P.: Novel media for detection of microbial producers of cellulase and xylanase.FEMS Microbiol. Letters 28, 137–140 (1985).
Kocková-Kratochvílová A., Sláviková E., Zemek J., Kadlečíková B., Kuniak L.: Utilization of the hydrolytic activity in taxonomy of hyphal yeastAscomycetes.Folia Microbiol. 28, 301–308 (1983).
Leathers T.D.: Color variants ofAureobasidium pullulans overproduce xylanase with extremely high specific activity.Appl. Environ. Microbiol. 52, 1026–1030 (1986).
Leathers T.D., Kurtzman C.P., Detroy R.W.: Overproduction and regulation of xylanase inAureobasidium pullulans andCryptococcus albidus.Biotechnol. Bioeng. Symp. 14, 225–240 (1984).
Paleg L.G.: Citric acid interference in the estimation of reducing sugars with alkaline copper reagent.Anal. Chem. 31, 1902–1904 (1959).
Paice M.G., Garnagul N., Page D.H., Jurasek L.: Mechanism of hemicellulose-directed prebleaching of kraft pulps.Enzyme Microb. Technol. 14, 272–276 (1992).
Puls J., Schuseil J.: Chemistry of hemicellulose: relationship between hemicellulose structure and enzymes required for hydrolysis, pp. 1–27 in M.P. Coughlan, G.P. Hazlewood:Hemicellulose and Hemicellulases. Portland Press, London 1993.
Suurnäkki A., Kantelinen A., Buchert J., Viikari L.: Enzyme-aided bleaching of industrial softwood kraft pulps.Tappi 11, 111–116 (1994).
Timell T.E.: Isolation of galactoglucomannans from the wood of gymnosperms.Tappi 2, 88–96 (1961).
Timell T.E.: Recent progress in the chemistry of wood hemicellulose.Wood Sci. Technol. 1, 45–70 (1967).
Viikari L., Ranua M., Kantelinen A., Linko M., Sandquist J.: Bleaching with enzymes, pp. 67–69 inProc. 3rd Int. Conf. Biotechnology in the Pulp and Paper industry, Stockholm 1986.
Viikari L., Ranua M., Kantelinen A., Linko M., Sandquist J.: Application of enzymes in bleachings, pp. 151–154 inProc. 4th Int. Symp. Wood and Pulping Chemistry, Vol. I, Paris 1987.
Wilkie K.C.B.: Hemicellulose.Chem. Technol. 306–319 (1983).
Wong K.K.Y., Sadler J.N.: Applications of hemicellulases in the food, feed, and pulp and paper industries, pp. 127–143 in M.P. Coughlan, G.P. Hazlewood:Hemicellulose and Hemicellulases. Portland Press, London 1993.
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Kremnický, L., Sláviková, E., Mislovičová, D. et al. Production of extracellular β-mannanases by yeasts and yeast-like microorganisms. Folia Microbiol 41, 43–47 (1996). https://doi.org/10.1007/BF02816339
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DOI: https://doi.org/10.1007/BF02816339