Abstract
Extracts of over 100 species of microalgae both from nature and from laboratory cultures were screened for antioxidant activity. As an assay for antioxidant function, we examined the inhibition of the activity of two oxidizing enzymes, lipoxygenase and tyrosinase. Water, ethanol, and methanol extracts of microalgae were used for the assay. It was found that lipoxygenase and tyrosinase activities were inhibited by the extract of several microalgae. Our results suggest that these species of microalgae have useful antioxidant activity, and they will be further examined for potential biotechnological exploitation. The aim of our screening was to identify potential sources of natural antioxidant compounds, which if found to be sufficiently potent and nontoxic, might eventually replace the chemicals that are currently used as food additives and cosmetics, in order to prevent food spoilage and their oxidation.
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References
Abeliovich, A. and Shilo, M. (1971),Isreal J. Med. Sci. 7, 9.
Abeliovich, A. and Shilo, M. (1972),J. Bacterial. 111, 682–689.
Bidigare, R. R., Ondrusek, M. E., Kennicutt, M. C. II., Iturriaga, R., Harvey, H. R., Hoham, R. W., and Macko, S. A. (1993),J. Phycol. 29, 427–434.
Sukenik, A., Zmara, O., and Cameli, Y. (1993),Aquaculture 117, 313–326.
Ohad, I., Adir, N., Koike, H., Kyle, D. J., and Inoue, Y. (1990),J. Biol. Chem. 265, 1972–1979.
Fridovich, I. (1978),Photochem. Photobiol. 28, 733–740.
Lesser, M. P. and Shick, J. M. (1989),Mar. Biol. 102, 243–255.
Borowitzka, M. A. and Borowitzka, L. J. (1988), inMicroalgal Biotechnology, Borowitzka, M. A., and Borowitzka, L. J., eds., Cambridge U.P., Cambridge, pp. 27–58.
Ben-Aziz, A. and Avron, M. (1989), inAlgal and Cyanobacterial Biotechnology, Cresswell, R. C., Rees, T. A. V, and Shah, N., eds., Longwan Scientistific and Technical Press, pp. 90–114.
Grobbelaar, J. U. (1995),J. Appl. Phycol. 7, 69–73.
Ben-Amotz, A. (1995),J. Appl Phycol. 7, 65–68.
Richmond, A. and Grobbelaar, J. U. (1986),Biomass 10, 253–264.
Borowitzka, M. A., Huisman, J. M., and Osborn, A. (1991)J. Appl. Phycol. 3, 295–304.
Boussiba, S. and Vonshak, A. (1991),Plant Cell Physiol. 32, 1077–1082.
Holman, R. T. (1951), The Enzymes, 1st ed., Academic, New York, p. 659.
Ben-Aziz, A., Grossman, S., Ascarelli, I., and Budowski, P. (1970),Anal. Biochem. 34, 88–100.
Lyckander, I. M. and Malterud, K. E. (1992),Acta Pharmaceutica Nordica 4, 159–166.
Wachi, Y., Burgess, J. G., Takahashi, J., Nakamura, N., and Matsunaga, T. (1995),J. Mar. Biotechnol. 2, 210–213.
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Matsukawa, R., Dubinsky, Z., Masaki, K. et al. Enzymatic screening of microalgae as a potential source of natural antioxidants. Appl Biochem Biotechnol 66, 239–247 (1997). https://doi.org/10.1007/BF02785590
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DOI: https://doi.org/10.1007/BF02785590