Abstract
Lentil cotyledonary nodes are some of the most regenerative tissues in legumes. Attempts to transform them by vacuum filtration have been limitedly successful. This report describes a rapid and convenient transient expression protocol based on vacuum infiltration ofAgrobacterium cells into lentil cotyledonary nodes. Vacuum-infiltrated tissues had significantly (P<.05) higher transient GUS expression than did noninfiltrated tissues. Under optimal conditions (infiltration at 200 mmHg for 20 min), 95% of theAgrobacterium-infiltrated explants exhibited an average of 16 blue foci. We believe this to be the first report of this technique for transient gene expression in lentil cotyledonary nodes.
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Abbreviations
- BAP :
-
6-Benzylaminopurine
- MES :
-
2-(N-morpholino) ethanesulfonic acid
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Mahmoudian, M., Yücel, M. & Öktem, H.A. Transformation of lentil (Lens culinaris M.) cotyledonary nodes by vacuum infiltration ofAgrobacterium tumefaciens . Plant Mol Biol Rep 20, 251–257 (2002). https://doi.org/10.1007/BF02782460
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DOI: https://doi.org/10.1007/BF02782460