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Proteins tightly bound with rDNA transcribed regions in nuclei, nucleoids, and nucleoproteins of human lymphocytes: Isolation and characterization of candidate proteins

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Abstract

Proteins tightly bound with the transcribed regions of human ribosomal genes protect them fromRsaI nuclease upon digestion of isolated lymphocyte nuclei, nucleoids, and DNP. Three proteins (p43, p52, p67) specifically binding with region 9346–10,783 of the 28S rRNA gene were isolated by affinity chromatography. Complexes of rDNA with p52 and p67 were stable under high ionic strength. Only p52 was covalently bound with the 28S rRNA gene region in lymphocytes exposed in UV light.

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Authors and Affiliations

  1. Institute of Human Genetics, Medical Genetic Research Center, Russian Academy of Medical Sciences, 115478, Moscow, Russia

    N. N. Veiko, N. A. Lyapunova, L. I. Kovalev, E. S. Ershova & D. M. Spitkovskii

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  1. N. N. Veiko
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  2. N. A. Lyapunova
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  3. L. I. Kovalev
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  4. E. S. Ershova
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  5. D. M. Spitkovskii
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Veiko, N.N., Lyapunova, N.A., Kovalev, L.I. et al. Proteins tightly bound with rDNA transcribed regions in nuclei, nucleoids, and nucleoproteins of human lymphocytes: Isolation and characterization of candidate proteins. Mol Biol 34, 250–255 (2000). https://doi.org/10.1007/BF02759647

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  • DOI: https://doi.org/10.1007/BF02759647

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