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High performance liquid chromatography analyses of emulsifiers: I. Quantitative determinations of mono- and diacylglycerols of saturated fatty acids

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Journal of the American Oil Chemists’ Society

Abstract

A high performance liquid chromatography (HPLC) method is described for the separation of mono-, di-, and triacylglycerols of fatty acids on a 25-cm column packed with 10 μm LiChrosorb DIOL. The acylglycerols are eluted isocratically with isooctane-isopropanol (95:5) within 10 min, and the components monitored by UV-absorption at 213 nm. The applicability of the method for the quantitative determination of the mono- and diacylglycerol content of fully hardened monoglycerides using an internal standard method has been demonstrated. The method shows excellent reproducibility and accuracy with standard deviations of a distilled monoglyceride containing 92.1% monoacylglycerols and 6.5% diacylglycerols of 0.70% and 0.42%, respectively. The method is applicable to other types of emulsifiers, for instance, acetylated monoglyceride emulsifiers and propylene glycol esters of fatty acids.

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Authors and Affiliations

  1. Grindsted Products Division, Edwin Rahrs Vej 38, DK-8220, Brabrand, Denmark

    T. Riisom & L. Hoffmeyer

Authors
  1. T. Riisom
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  2. L. Hoffmeyer
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Riisom, T., Hoffmeyer, L. High performance liquid chromatography analyses of emulsifiers: I. Quantitative determinations of mono- and diacylglycerols of saturated fatty acids. J Am Oil Chem Soc 55, 649–652 (1978). https://doi.org/10.1007/BF02682452

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  • DOI: https://doi.org/10.1007/BF02682452

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