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Properties of viruses of the potyvirus group. 3. A comparison of buoyant density, S value, particle morphology, and molecular weight of the coat protein subunit of 10 viruses and virus isolates

Eigenschappen van virussen van de potyvirusgroep. 3. Een vergelijking van de zweefdichtheid, de S-waarde, de deeltjesvorm en het molecuulgewicht van de mantele iwiteenheid van 6 isolaten van het bonescherpmozaïekvirus, het erwterolmozaïekvirus, twee isolaten van het aardappelvirus Y en het slamozaïekvirus

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Abstract

A comparison of six isolates of bean yellow mosaic virus, pea seed-borne mosaic virus, two isolates of potato virus Y, and lettuce mosaic virus revealed that differences exist with respect to buoyant density and S value, but that the differences between isolates of one virus can be as big as those between different viruses. There is no correlation between buoyant density and S value on the one hand and pathogenicity on the other hand. All viruses and virus isolates, except the potato virus Y isolates, show the Mg++ effect. The molecular weight of the coat protein subunits of all viruses was 34,000 daltons. In 8 out of 10 cases the protein was easily degraded into a 28,000 daltons component. Only the potato virus Y isolates did not show this effect.

Samenvatting

Uit een vergelijking van 10 virussen en virusisolaten van de aardappelvirus Y-groep bleek dat er weliswaar duidelijke verschillen bestaan met betrekking tot de zweefdichtheid en de S-waarde, maar dat de verschillen tussen isolaten van één virus even groot kunnen zijn als die tussen verschillende virussen. Er werd geen correlatie gevonden tussen zweefdichtheid en S-waarde enerzijds en pathogeniteit anderzijds.

Alle bestudeerde virussen en virusisolaten, met uitzondering van de aardap pelvirus Y-isolaten, vertoonden het Mg++-effect. Het molecuulgewicht van de man teleiwiteenheid bedraagt voor al de virussen 34.000 daltons. In 8 van de 10 gevallen werd het eiwit gemakkelijk omgezet in een component met een molecuulgewicht van 28.000 daltons. Alleen de aardappelvirus Y-isolaten vertoonden dit effect niet.

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References

  • Bokx, J. A. de, Kratchanova, B. & Maat, D. Z., 1975. Some properties of a deviating strain of potato virus Y. Potato Research 18: 38–51.

    Article  Google Scholar 

  • Bos, L., 1970. The identification of three new viruses fromWisteria andPisum in the Netherlands, and the problem of variation within the potato virus Y group. Neth. J. Pl. Path. 76: 8–46.

    Article  Google Scholar 

  • Bos, L., Kowalska, C. & Maat, D. Z., 1974. The identification of bean mosaic, pea yellow mosaic and pea necrosis strains of bean yellow mosaic virus. Neth. J. Pl. Path. 80: 173–191.

    Article  Google Scholar 

  • Geelen, J. L. M. C., Kammen, A. van & Verduin, B. J. M., 1972. Structure of the capsid of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology 49: 205–213.

    Article  PubMed  CAS  Google Scholar 

  • Govier, D. A. & Woods, R. D., 1971. Changes induced by magnesium ions in the morphology of some plant viruses with filamentous particles. J. gen. Virol. 13: 127–132.

    Article  PubMed  CAS  Google Scholar 

  • Harrison, B. D., Finch, J. T., Gibbs, A. J., Hollings, M., Shepherd, R. J., Valenta, V. & Wetter, C., 1971. Sixteen groups of plant viruses. Virology 45: 356–363.

    Article  PubMed  CAS  Google Scholar 

  • Huttinga, H., 1973. Properties of viruses of the potyvirus group. 1. A simple method to purify bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus and potato virus YN. Neth. J. Pl. Path. 79: 125–129.

    Article  CAS  Google Scholar 

  • Huttinga, H. & Mosch, W. H. M., 1974. Properties of viruses of the potyvirus group. 2. Buoyant density, S value, particle morphology, and molecular weight of the coat protein of bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus, and potato virus YN. Neth. J. Pl. Path. 80: 19–27.

    Article  Google Scholar 

  • Markham, R., 1960. A graphical method for the rapid determination of sedimentation coefficients. Biochem. J. 77: 516–519.

    PubMed  CAS  Google Scholar 

  • Mosch, W. H. M., Huttinga, H. & Rast, A. Th. B., 1973. Some chemical and physical properties of 18 tobacco mosaic virus isolates from tomato. Neth. J. Pl. Path. 79: 104–111.

    Article  CAS  Google Scholar 

  • Szybalski, W., 1968. Equilibrium sedimentation of viruses, nucleic acids and other macromolecules in density gradients. Fractions 1: 1–15.

    Google Scholar 

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  1. Instituut voor Plantenziektenkundig Onderzoek, Binnenhaven 12, Wageningen, the Netherlands

    H. Huttinga

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  1. H. Huttinga
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Huttinga, H. Properties of viruses of the potyvirus group. 3. A comparison of buoyant density, S value, particle morphology, and molecular weight of the coat protein subunit of 10 viruses and virus isolates. Netherlands Journal of Plant Pathology 81, 58–63 (1975). https://doi.org/10.1007/BF02650330

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  • DOI: https://doi.org/10.1007/BF02650330

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