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Structural properties of potexvirus coat proteins detected by optical methods

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Abstract

It has been shown by X-ray analysis that cores of coat proteins (CPs) from three potexviruses, flexible helical RNA-containing plant viruses, have similar α-helical structure. However, this similarity cannot explain structural lability of potexvirus virions, which is believed to determine their biological activity. Here, we used circular dichroism (CD) spectroscopy in the far UV region to compare optical properties of CPs from three potexviruses with the same morphology and similar structure. CPs from Alternanthera mosaic virus (AltMV), potato aucuba mosaic virus (PAMV), and potato virus X (PVX) have been studied in a free state and in virions. The CD spectrum of AltMV virions was similar to the previously obtained CD spectrum of papaya mosaic virus (PapMV) virions, but differed significantly from the CD spectrum of PAMV virions. The CD spectrum of PAMV virions resembled in its basic characteristics the CD spectrum of PVX virions characterized by molar ellipticity that is abnormally low for α-helical proteins. Homology modeling of the CP structures in AltMV, PAMV, and PVX virions was based on the known high-resolution structures of CPs from papaya mosaic virus and bamboo mosaic virus and confirmed that the structures of the CP cores in all three viruses were nearly identical. Comparison of amino acid sequences of different potexvirus CPs and prediction of unstructured regions in these proteins revealed a possible correlation between specific features in the virion CD spectra and the presence of disordered N-terminal segments in the CPs.

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Abbreviations

AltMV:

Alternanthera mosaic virus

BaMV:

bamboo mosaic virus

CD:

circular dichroism

CP:

coat protein

PAMV:

potato aucuba mosaic virus

PapMV:

papaya mosaic virus

PepMV:

pepino mosaic virus

PVX:

potato virus X

TMV:

tobacco mosaic virus

UV:

ultraviolet

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Authors and Affiliations

  1. Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, 119991, Moscow, Russia

    P. I. Semenyuk, A. L. Ksenofontov, N. O. Kalinina, E. N. Dobrov & V. V. Makarov

  2. Lomonosov Moscow State University, Biological Faculty, 119991, Moscow, Russia

    O. V. Karpova

Authors
  1. P. I. Semenyuk
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  2. O. V. Karpova
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  3. A. L. Ksenofontov
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  4. N. O. Kalinina
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  5. E. N. Dobrov
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  6. V. V. Makarov
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Corresponding authors

Correspondence to N. O. Kalinina or V. V. Makarov.

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Original Russian Text © P. I. Semenyuk, O. V. Karpova, A. L. Ksenofontov, N. O. Kalinina, E. N. Dobrov, V. V. Makarov, 2016, published in Biokhimiya, 2016, Vol. 81, No. 12, pp. 1838–1848.

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Semenyuk, P.I., Karpova, O.V., Ksenofontov, A.L. et al. Structural properties of potexvirus coat proteins detected by optical methods. Biochemistry Moscow 81, 1522–1530 (2016). https://doi.org/10.1134/S0006297916120130

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  • DOI: https://doi.org/10.1134/S0006297916120130

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