Abstract
The buoyant densities of bean yellow mosaic virus (BYMV) B25, pea mosaic virus (PMV) E198, lettuce mosaic virus (LMV), and potato virus YN (PVYN) were 1.318, 1.321, 1.330, and 1.326 g/ml, respectively. Their S values were 143, 140, 143, and 145 S. The particle morphology of BYMV B25, PMV E198, and LMV could reversibly be changed by magnesium ions. PVYN particles were broken in the presence of magnesium ions. The molecular weight of the coat protein subunit of the four viruses was 34,000 daltons. In many preparations also a 28,000 daltons component was present. This must be considered to be a breakdown product, derived from the 34,000 daltons component by proteolytic activity.
Samenvatting
De zweefdichtheden van het bonescherpmozaiekvirus (BYMV) B25, het erwtemozaiekvirus (PMV) E198, het slamozaïekvirus (LMV) en het aardappelvirus YN (PVYN) bedroegen respectievelijk 1,318, 1,321, 1,330 en 1,326 g/ml. Hun sedimentatiecoëfficiënten waren 143, 140, 143 en 145 S. De deeltjesvorm van BYMV B25, PMV E198 en LMV kon reversibel worden veranderd door toevoeging en verwijdering van magnesium-ionen. Deze induceerden het optreden van breuken in de deeltjes van PVYN. Het molecuulgewicht van de manteleiwiteenheid van de vier virussen bedroeg 34.000 daltons. In veel preparaten was bovendien een component van 28.000 daltons aanwezig. Deze moet worden beschouwd, als een proteolytisch afbraakprodukt dat ontstaat uit de component van 34.000 daltons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartels, R., 1964. Untersuchungen über serologische Beziehungen zwischen Viren der ‘tobacco-etch virus-Gruppe’. Phytopath. Z. 49: 257–265.
Bercks, R., 1961. Serologische Verwandschaft zwischen Kartoffel-Y-Virus, Rübenmosaik-Virus und Phaseolus-Virus 2. Phytopath. Z. 40: 357–365.
Bos, L., 1970. The identification of three new viruses fromWisteria andPisum in the Netherlands, and the problem of variation within the potato virus Y group. Neth. J. Pl. Path. 76: 8–46.
Brandes, J. & Wetter, C., 1959. Classification of elongated viruses on the basis of particle morphology. Virology 8: 99–115.
Brunt, A. A. & Kenten, R. H., 1971. Pepper veinal mottle-a new member of the potato virus Y group from peppers (Capsicum annuum L. andC. frutescens L.) in Ghana. Ann. appl. Biol. 69: 235–243.
Damirdagh, I. S. & Shepherd, R. J., 1970. Purification of the tobacco etch and other viruses of the potato Y group. Phytopathology 60: 132–142.
Geelen, J. L. M. C., Kammen, A. van & Verduin, B. J. M., 1972. Structure of the capsid of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology 49: 205–213.
Govier, D. A. & Plumb, R. T., 1972. Henbane mosaic virus. C.M.I./A.A.B. Descriptions of plant viruses nr. 95, pp. 4.
Govier, D. A. & Woods, R. D., 1971. Changes induced by magnesium ions in the morphology of some plant viruses with filamentous particles. J. gen. Virol. 13: 127–132.
Hill, J. H. & Shepherd, R. J., 1972a. Biochemical properties of turnip mosaic virus. Virology 47: 807–816.
Hill, J. H. & Shepherd, R. J., 1972b. Molecular weights of plant virus coat proteins by polyacrylamide gel electrophoresis. Virology 47: 817–822.
Huttinga, H., 1972. Interaction between long and short particles of tobacco rattle virus. Thesis, Wageningen.
Huttinga, H., 1973. Properties of viruses of the potyvirus group. 1. A simple method to purify bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus and potato virus YN. Neth. J. Pl. Path. 79: 125–129.
Koenig, R., Stegemann, H., Francksen, H. & Paul, H. L., 1970. Protein subunits in the potato virus X group. Determination of the molecular weights by polyacrylamide electrophoresis. Biochim. biophys. Acta 207: 184–189.
Markham, R., 1960. A graphical method for the rapid determination of sedimentation coefficients. Biochem. J. 77: 516–519.
Mayo, M. A. & Cooper, J. I., 1973. Partial degradation of the protein in tobacco rattle virus during storage. J. gen. Virol. 18: 281–289.
Niblett, C. L. & Semancik, J. S., 1969. Conversion of the electrophoretic forms of cowpea mosaic virus in vivo and in vitro. Virology 38: 685–693.
Purcifull, D. E. & Shepherd, R.J., 1964. Preparation of the protein fragments of several rod-shaped plant viruses and their use in agar-gel diffusion tests. Phytopathology 54: 1102–1108.
Rees, M. W. & Short, M. N., 1965. Variations in the composition of two strains of tobacco mosaic virus in relation to their host. Virology 26: 596–602.
Szybalski, W., 1968. Equilibrium sedimentation of viruses, nucleic acids and other macromolecules in density gradients. Fractions 1: 1–15.
Taylor, R. H. & Smith, P. R., 1968. The relationship between bean yellow mosaic virus and pea mosaic virus. Aust. J. biol. Sci. 21: 429–437.
Uyemoto, J. K., Provvidenti, R. & Schroeder, W. T., 1972. Serological relationship and detection of bean common and bean yellow mosaic viruses in agar gel. Ann. appl. Biol. 71: 235–242.
Weber, K. & Osborn, M., 1969. The reliability of molecular weight determinations by dodecyl sulfatepolyacrylamide gel electrophoresis. J. biol. Chem. 244: 4406–4412.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huttinga, H., Mosch, W.H.M. Properties of viruses of the potyvirus group. 2. Buoyant density, S value, particle morphology, and molecular weight of the coat protein subunit of bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus, and potato virus YN . Netherlands Journal of Plant Pathology 80, 19–27 (1974). https://doi.org/10.1007/BF01976427
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01976427