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Induction of microspore embryogenesis inBrassica napus L. by gamma irradiation and ethanol stress

  • Plant Cellular and Developmental Biology
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Summary

Gamma irradiation and ethanol stress treatments redirected pollen development to an embryo formation pathway inBrassica napus. Less than 0.01% of microspores developed into embryos at 25°C compared to approximately 2% at 32°C. However, subsequent to gamma irradiation and ethanol treatments up to 1% and 0.7% of microspores formed embryos at 25°C, respectively. Gamma irradiation also enhanced embryogenesis at 32°C. The possible importance of these findings is discussed in relation to microspore embryogenesis.

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Author notes

  1. P. M. Pechan

    Present address: Max Planck Institute für Züchtungsforschung, 5000, Köln 30, Federal Republic of Germany

Authors and Affiliations

  1. Plant Research Centre, Agriculture Canada, Bldg. 21, C.E.F., K1A 0C6, Ottawa, Ontario, Canada

    P. M. Pechan & W. A. Keller

Authors
  1. P. M. Pechan
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  2. W. A. Keller
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Pechan, P.M., Keller, W.A. Induction of microspore embryogenesis inBrassica napus L. by gamma irradiation and ethanol stress. In Vitro Cell Dev Biol 25, 1073–1074 (1989). https://doi.org/10.1007/BF02624144

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  • DOI: https://doi.org/10.1007/BF02624144

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