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Organ cultures of rat and hamster colon

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Summary

Human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. Colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. Explants of rat and hamster colon, maintained in Leibovitz's L-15 medium buffered with HEPES in the presence of 5% CO2 and air, retained their normal columnar epithelial architecture for 24 days in culture. Outgrowth of epithelial cells from colonic explants similar to those of the mother fragments grew into the foam. Viability of the explants was indicated by radioactive thymidine incorporation, and by the ability of the fragments to support viral replication.

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References

  1. Leighton, J. 1951. A sponge matrix method for tissue culture. Formation of organized aggregates of cells in vitro. J. Natl. Cancer Inst. 12: 545–561.

    PubMed  CAS  Google Scholar 

  2. Leighton, J., G. Justh, M. Esper, and R. L. Kronenthal. 1967. Collagen-coated cellulose sponge: three-dimensional matrix for tissue culture of Walker tumor 256. Science 155: 1259–1261.

    Article  PubMed  CAS  Google Scholar 

  3. Kalus, M., and V. Klement. 1966. Cultivation of organ fragments on fibrin foam. Folia Biol. (Praha) 12: 468–472.

    CAS  Google Scholar 

  4. Kalus, M., and R. M. O'Neal. 1968. Organ tissue culture on a three-dimensional matrix of human fibrin foam. Arch. Pathol. 86: 52–59.

    PubMed  CAS  Google Scholar 

  5. Leighton, J., R. Mark, and G. Justh. 1968. Patterns of three-dimensional growth in vitro in collagen-coated cellulose sponge: carcinomas and embryonic tissues. Cancer Res 28: 286–296.

    PubMed  CAS  Google Scholar 

  6. Good, N. E., G. D. Winget, W. Winter, T. N. Connolly, S. Izawa, and R. M. M. Singh. 1966. Hydrogen ion buffers for biological research. Biochemistry 5: 467–477.

    Article  PubMed  CAS  Google Scholar 

  7. Roller, M.-R., S. P. Owen, and C. Heidelberger. 1966. Studies on the organ culture of human tumors. Cancer Res. 26: 626–637.

    PubMed  CAS  Google Scholar 

  8. Kalus, M., J. J. Ghidoni, and R. M. O'Neal. 1968. The growth of tumors in matrix cultures. Cancer 22: 507–516.

    Article  PubMed  CAS  Google Scholar 

  9. Kalus, M. 1972. Carcinoma and adenomatous polyps of the colon and rectum in biopsy and organ tissue culture. Cancer 30: 972–982.

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Life Sciences Research Division, IIT Research Institute, 10 West 35th St., 60616, Chicago, Ill

    L. J. Schiff

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  1. L. J. Schiff
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Additional information

This investigation was supported by IIT Research Institute under Project L1062

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Schiff, L.J. Organ cultures of rat and hamster colon. In Vitro 11, 46–49 (1975). https://doi.org/10.1007/BF02615321

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  • DOI: https://doi.org/10.1007/BF02615321

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