Skip to main content

Advertisement

SpringerLink
Log in

The metabolism of labeled parathyroid hormone

V. Collected biological studies

  • Original Papers
  • Published:
Calcified Tissue Research Aims and scope Submit manuscript

Abstract

Biologically active125I-labeled parathyroid hormone (125I-PTH) was used in a series of studies in dogs and chickens designed to confirm and augment earlier studies in rats. As in rats, a three exponential equation was required to describe disappearance of125I-PTH from the blood in the dog. The first two “half-lives” (1.8 and 7 min) accounted for the bulk of the dose. Also as in rats, deposition of apparently intact hormone took place rapidly in kidney, liver and bone in both the dog and the chicken. Degradation occurred very rapidly in all three target organs. Three labeled hormones of different biological activities were compared in the rat. Inactive, oxidized hormone was rejected by the liver but showed markedly increased deposition in kidney and the higher the purity of the hormone the higher was its uptake by liver. Exploration of a wide range of dosages revealed few effects on distribution (smaller deposition in liver and kidney at highest dosages, 65 μg/rat). Fresh sera did not degrade hormone rapidly or extensively. There was no deposition of hormone in intestinal mucosa, marrow, and red cells. Nephrectomy increased deposition in liver and bone. Finally, the perfused liver was capable of extensive degradation of the hormone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altman, P. L., Dittmer, D. S.: Blood and other body fluids, p. 4–7. Washington, D. C.: Fed. Amer. Soc. exp. Biol. 1961

    Google Scholar 

  2. Arnaud, C. D., Sizemore, G. W., Oldham, S. B., Fischer, J. A., Tao, H. S., Littledike, E. T.: Human parathyroid hormone: glandular and secreted species. Amer. J. Med.50, 630–638 (1971)

    Article  PubMed  CAS  Google Scholar 

  3. Brunette, M. G., Taleb, L., Carriere, S.: Effect of parathyroid hormone on phosphate reabsorption along the nephron of the rat. Amer. J. Physiol.225, 1076–1081 (1973)

    PubMed  CAS  Google Scholar 

  4. Canterbury, J. M., Levey, G. S., Reiss, E.: Activation of renal cortical adenylate cyclase by circulating immunoreactive parathyroid hormone fragments. J. clin. Invest.52, 524–527 (1973)

    Article  PubMed  CAS  Google Scholar 

  5. Chase, L. R., Aurbach, G. D.: Cyclic AMP and the mechanism of action of parathyroid hormone. In: Parathyroid hormone and thyrocalcitonin, (Talmage, R. V., Belanger, L. F., eds.), p. 247–257, Amsterdam: Excerpta Medica Foundation 1968

    Google Scholar 

  6. Cohn, D. V., Macgregor, R. R., Chu, L. L. H., Kimmel, J. R.: Calcemic fraction A: Biosynthetic peptide precursor of parathyroid hormone. Proc. nat. Acad. Sci. (Wash.)69, 1521–1525 (1972)

    Article  CAS  Google Scholar 

  7. Cox, A. C., Balloun, S. L.: Prediction of total skeletal mineralization from individual bone data. Poultry Sci.50, 186–194 (1971)

    CAS  Google Scholar 

  8. deLuise, M., Martin, T. J., Melick, R. A.: Tissue distribution of calcitonin in the rat: comparison with parathyroid hormone. J. Endocrinol.48, 173–179 (1970)

    Article  CAS  Google Scholar 

  9. DiBella, F. P., Dousa, T. P., Miller, S. S., Arnaud, C. D.: Parathyroid hormone receptors of renal cortex: specific binding of biologically active125I-labeled hormone and relationship to adenylate cyclase activation. Proc. nat. Acad. Sci. (Wash.)71, 723–726 (1974)

    Article  CAS  Google Scholar 

  10. Fang, V. S., Tashjian, A. H., Jr.: Studies on the role of the liver in the metabolism of parathyroid hormone. I. Effects of partial hepatectomy and incubation of the hormone with tissue homogenates. Endocrinol.90, 1177–1184 (1972)

    CAS  Google Scholar 

  11. Fischer, J. A., Oldham, S. B., Sizemore, G. W., Arnaud, C. D.: Calcium-regulated parathyroid hormone peptidase. Proc. nat. Acad. Sci. (Wash.)69, 2341–2345 (1972)

    Article  CAS  Google Scholar 

  12. Fujita, T., Orimo, H., Ohata, M., Maruyama, M.: Enzymatic inactivation of parathyroid hormone by rat kidney homogenate. Endocrinol.86, 42–49 (1970)

    CAS  Google Scholar 

  13. Galante, L., Colston, K., Evans, I. M. A., Byfield, P. G. H., Matthews, E. W., MacIntyre, I.: The regulation of vitamin D metabolism. Nature (Lond.)244, 438–440 (1973)

    Article  CAS  Google Scholar 

  14. Galante, L., Colston, K., MacAuley, S., MacIntyre, I.: Effect of parathyroid extract on vitamin D metabolism. Lancet May6, 985–988 (1972)

    Article  Google Scholar 

  15. Habener, J. F., Kemper, B., Potts, J. T., Jr., Rich, A.: Proparathyroid hormone: biosynthesis by human parathyroid adenomas. Science178, 630–633 (1972)

    Article  PubMed  CAS  Google Scholar 

  16. Habener, J. F., Powell, D., Murray, T. M., Mayer, G. P., Potts, J. T., Jr.: Parathyroid hormone: secretion and metabolismin vivo. Proc. nat. Acad. Sci. (Wash.)68, 2986–2991 (1971)

    Article  CAS  Google Scholar 

  17. Habener, J. F., Segre, G. V., Powell, D., Murray, T. M., Potts, J. T., Jr.: Immunoreactive parathyroid hormone in circulation of man. Nature (Lond.) New Biol.238, 152–154 (1972)

    CAS  Google Scholar 

  18. Hamilton, J. W., Cohn, D. V.: Studies on the biosynthesisin vitro of parathyroid hormone. J. biol. Chem.244, 5421–5429 (1969)

    PubMed  CAS  Google Scholar 

  19. Hamilton, J. W., Macgregor, R. R., Chu, L. L. H., Cohn, D. V.: The isolation and partial purification of a non-parathyroid hormone calcemic fraction from bovine parathyroid glands. Endocrinol.89, 102–109 (1971)

    Google Scholar 

  20. John, D. W., Miller, L. L.: Regulation of net biosynthesis of serum albumin and acute phase plasma proteins. J. biol. Chem.244, 6134–6142 (1969)

    PubMed  CAS  Google Scholar 

  21. Kemper, B., Habener, J. F., Potts, J. T., Jr., Rich, A.: Proparathyroid hormone: Identification of a biosynthetic precursor to parathyroid hormone. Proc. nat. Acad. Sci. (Wash.)69, 643–647 (1972)

    Article  CAS  Google Scholar 

  22. Keutmann, H. T., Aurbach, G. D., Dawson, B. F., Niall, H. D., Deftos, L. J., Potts, J. T., Jr.: Isolation and characterization of bovine parathyroid hormone isohormones. Biochem.10, 2779 (1971)

    Article  CAS  Google Scholar 

  23. Lattimore, H. B.: Postnatal growth of the chicken skeleton. Amer. J. Anat.40, 1–58 (1927)

    Article  Google Scholar 

  24. Malbon, C. C., Zull, J. E.: Interactions of parathyroid hormone and plasma membranes from rat kidney. Biochem. biophys. Res. Commun.56, 952–958 (1974)

    Article  PubMed  CAS  Google Scholar 

  25. Martin, T. J., Greenberg, P. B., Micheangeli: Synthesis of human parathyroid hormone by cultured cells: evidence for release of prohormone by some adenomata. Clin. Sci.44, 1–8 (1973)

    PubMed  CAS  Google Scholar 

  26. Martin, T. J., Greenberg, P., Melick, R. A.: Nature of human parathyroid hormone secreted by monolayer cell cultures. J. clin. Endocr.34, 437–440 (1972)

    PubMed  CAS  Google Scholar 

  27. Melick, R. A., Aurbach, G. D., Potts, J. T., Jr.: Distribution and half-life of131I-labeled parathyroid hormone in the rat. Endocrinol.77, 198–202 (1965)

    CAS  Google Scholar 

  28. Moxley, M. A., Bell, N. H., Ashmore, J.: Stimulation of 3′,5′-cyclic adenosinemonophosphate (cAMP) glucose and urea production by parathyroid hormone in isolated rat hepatocytes. Fed. Proc.33, 544 (1974)

    Google Scholar 

  29. Neuman, W. F.: The Mileau Interieur of bone: Claude Bernard revisited. Fed. Proc.28, 1846–1850 (1969)

    PubMed  CAS  Google Scholar 

  30. Neuman, W. F., Neuman, M. W., Sammon, P. J., Casarett, G. W.: The metabolism of labeled parathyroid hormone. IV. Autoradiographic studies. Calcif. Tiss. Res.18, 263–270 (1975)

    CAS  Google Scholar 

  31. Neuman, W. F., Neuman, M. W., Sammon, P. J., Lane, K.: The metabolism of labeled parathyroid hormone. II. Methodological studies. Calcif. Tiss. Res.18, 241–250 (1975)

    CAS  Google Scholar 

  32. Neuman, W. F., Neuman, M. W., Sammon, P. J., Simon, W., Lane, K.: The metabolism of labeled parathyroid hormone. III. Studies in rats. Calcif. Tiss. Res.18, 251–261 (1975)

    CAS  Google Scholar 

  33. Potts, J. T., Jr., Keutmann, H. T., Niall, H. D., Habener, J. F., Tregear, G. W., Deftos, L. J., O’Riordan, J. L. H., Aurbach, G. D.: Chemistry of the parathyroid hormones: clinical and physiological implications. In: Calcium parathyroid hormone and the calcitonins (Talmage, R. V., Munson, P. L., eds.), p. 159–172. Amsterdam: Excerpta Medica 1972

    Google Scholar 

  34. Potts, J. T., Jr., Niall, H. D., Keutmann, H. T., Lequin, R. M.: Chemistry of the calcitonins: species variation plus structure-acrivity relations and phyrmacologic implications. In: Calcium parathyroid hormone and the calcitonins (Talmage, R. V., Munson, P. L., eds.), p. 121–127. Amsterdam: Excerpta Medica 1972

    Google Scholar 

  35. Potts, J. T., Jr., Tregear, G. W., Keutmann, H. T., Niall, H. D., Sauer, R., Dawson, B. F., Hogan, M. L., Aurbach, G. D.: Synthesis of biologically active N-terminal tetratriacontapeptide of parathyroid hormone. Proc. nat. Acad. Sci. (Wash.)68, 63–67 (1971)

    Article  CAS  Google Scholar 

  36. Potts, J. T., Jr., Niall, H. D., Tregear, G. W., VanRietschoten, J., Habener, S. F., Segre, G. V., Keutmann, H. T.: Chemical and biological studies of proparathyroid hormone and parathyroid hormone: Analysis of hormone biosynthesis and metabolism. Mt Sinai J. Med.40, 448–461 (1973)

    PubMed  CAS  Google Scholar 

  37. Sammon, P. J., Brand, J. S., Neumann, W. F., Raisz, L. G.: The metabolism of parathyroid hormone. I. Preparation of biologically active125I-labeled parathyroid hormone. Endocrinol.92, 1596–1603 (1973)

    CAS  Google Scholar 

  38. Sherwood, L. J., Rodman, J. S., Lundberg, W. B.: Evidence for a precursor to circulating parathyroid hormone. Proc. nat. Acad. Sci. (Wash.)67, 1631–1638 (1970)

    Article  CAS  Google Scholar 

  39. Skelton, H.: The storage of water by various tissues of the body. Arch. intern. Med.40, 140–152 (1972).

    Google Scholar 

  40. Sutcliffe, H. S., Martin, T. J., Eisman, J. A., Piczyk, R.: Binding of parathyroid hormone to bovine kidney cortex plasma membranes. Biochem. J.134, 913–921 (1973).

    PubMed  CAS  Google Scholar 

  41. Tregear, G. W., VanRietschoten, J., Greene, E., Keutmann, H. T., Niall, H. D., Reit, B., Parsons, J. A., Potts, J. T., Jr.: Bovine parathyroid hormone: minimum chain length of synthetic peptide required for biological activity. Endocrinol.93, 1349–1353 (1973)

    CAS  Google Scholar 

  42. Vajda, F. J. E., Martin, T. J., Melick, R. A.: Destruction of bovine parathyroid hormone labeled with131I by rat kidney tissue. Endocrinol.84, 162–164 (1969)

    Article  CAS  Google Scholar 

  43. Zull, J. E., Repke, D. W.: The tissue localization of tritiated parathyroid hormone in thyroparathyroidectomized rats. J. biol. Chem.247, 2195–2199 (1972)

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. P. J. Sammon

    Present address: Department of Oral Biology, University of Kentucky, College of Dentistry, 40506, Lexington, Kentucky, USA

Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, School of Medicine and Dentistry, University of Rochester, 14642, Rochester, New York, USA

    W. F. Neuman, M. W. Neuman, K. Lane, L. Miller & P. J. Sammon

Authors
  1. W. F. Neuman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. W. Neuman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. J. Sammon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Neuman, W.F., Neuman, M.W., Lane, K. et al. The metabolism of labeled parathyroid hormone. Calc. Tis Res. 18, 271–287 (1975). https://doi.org/10.1007/BF02546246

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02546246

Key words

Search

Navigation