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Determination of triglyceride structure via silver nitrate-TLC

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

A procedure for the quantitative analysis of component triglycerides by thin-layer chromatography (TLC) with silver nitrate-silicic acid coated plates in combination with lipase hydrolysis is described. The method is demonstrated on synthetic triglyceride mixtures and applied to several natural fats.

The results indicate that the fatty acids of natural fats are not distributed among the triglycerides in accordance with any simple mathematical formula and methods based on mathematical distribution patterns cannot be generally applied.

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Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, Austin, Minnesota

    M. L. Blank, B. Verdino & O. S. Privett

Authors
  1. M. L. Blank
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  2. B. Verdino
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  3. O. S. Privett
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Additional information

Supported in part by a grant from the Special Dairy Industry Board of the National Dairy Council and U.S. Public Health Service Grant No. HE 05735.

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Blank, M.L., Verdino, B. & Privett, O.S. Determination of triglyceride structure via silver nitrate-TLC. J Am Oil Chem Soc 42, 87–90 (1965). https://doi.org/10.1007/BF02545035

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  • DOI: https://doi.org/10.1007/BF02545035

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