Abstract
The metabolism of lysophosphatidylcholine (LPC) in non-ischemic and ischemic canine heart was investigated byin vitro enzyme analysis. Selected subcellular fractions were assayed for the LPC-producing enzyme phospholipase A and the LPC-eliminating enzymes LPC:acyl-CoA acyltransferase, LPC:LPC transacylase and lysophospholipase. The canine heart was found to contain all enzymes differing, however, in subcellular distribution and specific activity. Phospholipase A activity did not change significantly in any of the fractions prepared from the ischemic tissue of hearts rendered ischemic for 1, 3 or 5 hr when compared to non-ischemic tissue. Changes in the activity of the microsomal LPC:acyl-CoA acyltransferase over the course of 5 hr of ischemia were observed. Significant decreases in the activity of the cytosolic and microsomal lysophospholipases were detected especially after 3 and 5 hr of ischemia. Similarly, a decrease in the activity of the microsomal LPC:LPC transacylase was noted after 3 and 5 hr of ischemia. Our results suggest that impaired catabolism of LPC rather than an enhanced production of LPC is the principal mechanism for the increase in LPC levels in the ischemic canine heart.
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Abbreviations
- ANOVA:
-
analysis of variance
- FA:
-
fatty acid
- LPC:
-
lysophosphatidylcholine
- PC:
-
phosphatidylcholine
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Mock, T., Man, R.Y.K. Mechanism of lysophosphatidylcholine accumulation in the ischemic canine heart. Lipids 25, 357–362 (1990). https://doi.org/10.1007/BF02537977
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DOI: https://doi.org/10.1007/BF02537977