Abstract
The destruction of cytochrome c during its catalysis of the lipoperoxidase reaction, which causes the impairment of mitochondrial membranes in living cells and the release of various pro-apoptotic factors into the cytosol, was studied. Spectrophotometric analysis showed a much more intense destruction of cytochrome c when hydrogen peroxide was added in the presence of tetraoleylcardiolipin at protein : cardiolipin ratios of 1 : 30 and 1 : 60, which were optimal for the formation of a cytochrome c complex with cardiolipin, compared to the sample in which only hydrogen peroxide and cytochrome c were present. In the second case, the destruction of the porphyrite group of heme took the form of a linear function, while in the presence of cardiolipin the dependence was clearly exponential; upon addition of a lipid substrate, phosphatidic acid, the value of the first-order rate constant of the cytochrome c destruction increased. It is proposed that the rapid destruction of cytochrome c during its catalysis of the lipoperoxidase reaction is an evolutionarily developed mechanism for preventing the spontaneous initiation of apoptosis.
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Funding
The experiments whose results are presented in this paper were supported by the Russian Foundation for Basic Research, project no. 18-015-00491 Study of the mechanism of reactions of free radical formation in the membranes of cells and mitochondria catalyzed by the cytochrome c complex with anionic lipids (Cyt−AL).
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Translated by E. Puchkov
Abbreviations: CytC, cytochrome c; PA, phosphatidic acid; TOCL, tetraoleylcardiolipin; CytC−TOCL, complex of cytochrome c with tetraoleylcardiolipin.
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Romodin, L.A., Vladimirov, Y.A. & Lysenko, N.P. The Destruction of Cytochrome c in a Complex with Cardiolipin during Catalysis of Lipid Peroxidation. BIOPHYSICS 66, 59–64 (2021). https://doi.org/10.1134/S0006350921010085
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DOI: https://doi.org/10.1134/S0006350921010085