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Molecular species composition of glycerophospholipids from white matter of human brain

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Lipids

Abstract

The molecular species composition of the major glycerophospholipids from white matter of human brain were determined by high-performance liquid chromatography of the 3,5-dinitrobenzoyl derivatives of the corresponding diradylglycerols. In phosphatidylcholine (PC) and phosphatidylserine (PS), molecular species containing only saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) comprised 85.7 and 82.4% of the respective totals, with 18∶0/18∶1 predominant in PS and 16∶0/18∶1 in PC. These molecular species were also abundant in phosphatidylethanolamine (PE), but in this phospholipid species containing polyunsaturated fatty acids (PUFA), largely 18∶0/22∶6n−3 and 18∶0/20∶4n−6, accounted for over half the total; 18∶1/18∶1 was also abundant in PE. In contrast, 1-O-alk-1′-enyl-2-acylsn-glycero-3-phosphoethanolamine (GPE) had much more SFA- and MUFA-containing species, predominantly 16∶0a/18∶1, 18∶0a/18∶1 and 18∶1a/18∶1, with low amounts of species containing 20∶4n−6 and 22∶6n−3. In alkenylacyl GPE, 22∶4n−6 was the major PUFA and 16∶0a/22∶4n−6 and 18∶1a/22∶4n−6 the main PUFA-containing species. There was six times more 22∶6n−3, twice as much 20∶4n−6 and half the amount of 22∶4n−6 in PE as compared to alkenylacyl GPE.

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Abbreviations

BHT:

butylated hydroxytoluene

GLC:

gas-liquid chromatography

GPE:

sn-glycero-3-phosphoethanolamine

HPLC:

high-performance liquid chromatography

HPTLC:

high-performance thin-layer chromatography

MUFA:

monounsaturated fatty acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

SFA:

saturated fatty acid

TLC:

thin-layer chromatography

UV:

ultraviolet

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Authors and Affiliations

  1. Department of Biological and Molecular Sciences, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland

    Robert Wilson

  2. NERC Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland

    Michael V. Bell

Authors
  1. Robert Wilson
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  2. Michael V. Bell
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Additional information

Molecular species are abbreviated as follows:e.g., 16∶0/18∶1 PE is 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine; the corresponding alkenylacyl species, 1-O-hexadec-1′-enyl-2-oleoyl-sn-glycero-3-phosphoethanolamine is 16∶0a/18∶1.

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Wilson, R., Bell, M.V. Molecular species composition of glycerophospholipids from white matter of human brain. Lipids 28, 13–17 (1993). https://doi.org/10.1007/BF02536353

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  • DOI: https://doi.org/10.1007/BF02536353

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