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The acyl and Alk-1-enyl groups of the major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions

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Lipids

Abstract

The major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions were separated by preparative thin layer chromatography. Alk-1-enyl groups from the alk-1-enyl acyl glycerophosphorylethanolamines were reacted with 1,3-propanediol to form the 1,3-dioxolane derivatives. Acyl groups were converted to the methyl ester derivatives and the acyl groups from alk-1-enyl acyl glycerophosphorylethanolamines and diacyl glycerophosphorylethanolamines were also determined separately. The acyl and alk-1-enyl group compositions of the phosphoglycerides from microsomal and mitochondrial fractions were quite similar. The ethanolamine and serine phosphoglycerides contained large amounts of 18∶0, 18∶1, 20∶4 and 22∶6 acyl groups. The choline phosphoglycerides had small amounts of polyunsaturated acyl groups and large amounts of 16∶0, 18∶1 and 18∶0 acyl groups. The mitochondrial cardiolipins contained unusual amounts of several acyl groups including 18∶1, 52%; 18∶2, 6%; and 16.1, 4%. A large portion of the mouse brain 18∶2 is in that fraction. The myelin phosphoglycerides were deficient in saturated and 22∶6 groups and markedly enriched in 18∶1 and 20∶1 groups when compared with the corresponding microsomal or mitochondrial phosphoglycerides.

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Author notes

  1. Lloyd A. Horrocks

    Present address: Laboratory of Neurochemistry, Cleveland Psychiatric Institute, 44109, Cleveland, Ohio

Authors and Affiliations

  1. Department of Physiological Chemistry, The Ohio State University, 410 West Tenth Avenue, 43210, Columbus, Ohio

    Grace Y. Sun & Lloyd A. Horrocks

Authors
  1. Grace Y. Sun
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  2. Lloyd A. Horrocks
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Additional information

Presented in part at the AOCS Meetings, New York, October 1968 and San Francisco, April 1969.

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Sun, G.Y., Horrocks, L.A. The acyl and Alk-1-enyl groups of the major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions. Lipids 5, 1006–1012 (1970). https://doi.org/10.1007/BF02533205

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  • DOI: https://doi.org/10.1007/BF02533205

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