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The relationship between fatty acid peroxidation and α-tocopherol consumption in isolated normal and transformed hepatocytes

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Lipids

Abstract

The response of normal and transformed rat hepatocytes to oxidative stress was investigated. Isolated normal rat hepatocytes and differentiated hepatoma cells (the Fao cell line was derived from the Reuber H 35 rat hepatoma) in suspension were incubated with the ADP/Fe3+ chelate for 30 min at 37°C. Membrane lipid oxidation was assessed by measuring (i) free malondialdehyde (MDA) production by a high-performance liquid chromatography (HPLC) procedure, (ii) membrane fatty acid disappearance as judged by capillary gas chromatography, and (iii) α-tocopherol oxidation as determined by HPLC and electrochemical detection. The addition of iron led to increased MDA production in normal as well as in transformed cells, and to simultaneous consumption of polyunsaturated fatty acids (PUFA) and α-tocopherol. In addition, in Fao cells more α-tocopherol was consumed during lipid peroxidation while less PUFA was oxidized. Lipid peroxidation was lower in tumoral hepatocytes than in normal cells. This could be due to a difference in membrane lipid composition because of a lower PUFA content and a higher α-tocopherol level in Fao cells. During oxidation, Fao cells produced 1.5 to 2 times less MDA than normal cells, while in the tumoral cells the amount of oxidized PUFA having 3 or more double bonds was 7 to 8 times lower. Therefore, measuring MDA alone as an index of lipid peroxidation did not allow for proper comparison of the membrane lipid oxidizability of transformed cellsvs. the membrane lipid oxidizability of normal cells.

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Abbreviations

ADP:

adenosine diphosphate

BHT:

butylated hydroxytoluene

FA:

fatty acid

FAME:

fatty acid methyl ester

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

MDA:

malondialdehyde

PUFA:

polyunsaturated fatty acid

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Faculty of Pharmacy, Cellular Biology and Botany Laboratory, 35043, Rennes Cedex, France

    Pascale Cogrel, Isabelle Morel, Martine Chevanne, Pierre Cillard & Josiane Cillard

  2. Liver Research Unit, INSERM U49, Ponchaillou Hospital, 35033, Rennes Cedex, France

    Gerard Lescoat & Pierre Brissot

Authors
  1. Pascale Cogrel
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  2. Isabelle Morel
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  3. Gerard Lescoat
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  4. Martine Chevanne
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  5. Pierre Brissot
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  6. Pierre Cillard
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  7. Josiane Cillard
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Cogrel, P., Morel, I., Lescoat, G. et al. The relationship between fatty acid peroxidation and α-tocopherol consumption in isolated normal and transformed hepatocytes. Lipids 28, 115–119 (1993). https://doi.org/10.1007/BF02535774

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  • DOI: https://doi.org/10.1007/BF02535774

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