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A comparison of lipids from liver and hepatoma subcellular membranes

  • Papers from the Brian L. Walker Memorial Symposium on Lipids and Cancer Presented at the 76th AOCS Annual Meeting in Philadelphia, Pennsylvania, May 1985
  • Published:
Lipids

Abstract

Subcellular fractions of nuclei, mitochondria, endoplasmic reticulum, plasma membrane and cytosol were prepared from liver and hepatoma 72288CTC. Marker enzyme activities, biochemical compositions and electron microscopy were used to establish purity. Hepatoma NADH: cytochrome C reductase and 5′-nucleotidase exhibited abnormal subcellular distributions.

The lipids from the subcellular fractions were examined in detail. Mitochondria and plasma membranes were characterized by elevated percentages of diphosphatidylglycrerol and sphingomyelin, respectively, in both tissues. All hepatoma subcellular fractions contained dramatically elevated levels of sphingomyelin and cholesterol, two components that form preferential strong complexes in vitro. The fatty acid composition of hepatoma sphingomyelin differed markedlg from liver and, unlike liver, did not exhibit organelle specific compositions. Some hepatoma lipid classes contained reduced percentages of palmitate while others contained higher levels. Hepatoma phosphatidylcholine and phosphatidylethanolamine from organelles contained lower percentages of long chain polyunsaturated fatty acids than liver. Generally, unique fatty acid profiles exhibited by individual phospholipid classes of liver subcellular fractions were absent or much reduced in the hepatoma. The ratios of oleate to vaccenate were near one for most of the phospholipid classes of most liver fractions, but all hepatoma classes, with few exceptions, contained a much higher percentage of oleate in all subcellular fractions. The hypothesis is proposed that the origin of some acyl moieties for the biosynthesis of various hepatome lipid classes differs from liver sources. The possible changes in acyl pools, sources and compartments for complex lipid biosynthesis could result in change in the quantities of molecular species that could contribute to the abnormal properties of the hepatoma membranes.

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References

  1. Wallach, D.F.H. (1968)Proc. Natl. Acad. Sci. 61, 868–874.

    Article  PubMed  CAS  Google Scholar 

  2. Wood, R. (1973) inTumor Lipids: Biochemistry and Metabolism (Wood, R., ed.) American Oil Chemists' Society, Champaign, IL.

    Google Scholar 

  3. Wood, R., Falch, J., and Wiegand, R.D. (1975)Lipids 10, 202–207.

    Article  PubMed  CAS  Google Scholar 

  4. Wood, R. (1975)Lipids 10, 736–745.

    Article  PubMed  CAS  Google Scholar 

  5. Wood, R., and Wiegand, R.D. (1975)Lipids 10, 746–749.

    Article  PubMed  CAS  Google Scholar 

  6. Wood, R., Chumbler, F., and Wiegand, R. (1977)J. Biol. Chem. 252, 1965–1970.

    PubMed  CAS  Google Scholar 

  7. Ruggieri, S., and Fallani, A. (1979)Lipids 14, 781–788.

    Article  PubMed  CAS  Google Scholar 

  8. Ruggieri, S., and Fallani, A. (1979)Lipids 14, 323–333.

    Article  PubMed  CAS  Google Scholar 

  9. Van Hoeven, R.P., and Emmelot, P. (1972)J. Membrane Biol 9, 105–126.

    Article  Google Scholar 

  10. Koizumi, K., Ito, Y., Koyima, K., and Fujii, T. (1976)J. Biochem. 79, 739–748.

    PubMed  CAS  Google Scholar 

  11. Bergelson, L.D., Dyatloviskaya, E.V., Torkhovskaye, T.I., Sorokina, L.B. and Gorkova, N.P. (1970)Biochim. Biophys. Acta 210, 287–298.

    PubMed  CAS  Google Scholar 

  12. Upreti, G.C., deAntueno, R.J., and Wood, R. (1983)J. Natl. Cancer. Inst. 70, 559–566.

    PubMed  CAS  Google Scholar 

  13. Upreti, G.C., deAntueno, R.J., and Wood, R. (1983)J. Natl. Cancer Inst. 70, 567–573.

    PubMed  CAS  Google Scholar 

  14. Wallach, D.F.H., and Kamat, V.B. (1966) inMethods of Enzymology. (Neufeld, E., and Ginsburg, V., eds.) Vol. VIII, pp. 164–172, Academic Press, New York.

    Google Scholar 

  15. Allfrey, V.G., Littau, V.C., and Mirsky, A.E. (1964)J. Cell Biol. 21, 213–231.

    Article  PubMed  CAS  Google Scholar 

  16. Fleischer, S., McIntyre, J.O., and Vidal, J.C. (1979) inMethods of Enzymology (Fleischer, S., and Packer, L., eds.) Vol. 55, pp. 32–39, Academic Press, New York.

    Google Scholar 

  17. Fleischer, S., and Kervina, M. (1974) inMethods of Enzymology (Fleischer, S., and Packer, L., eds.) Vol. 31, pp. 6–41, Academic Press, New York.

    Google Scholar 

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  19. Rouser, G., Siakotos, A.N., and Fleischer, S. (1966)Lipids 1, 85–87.

    Article  CAS  PubMed  Google Scholar 

  20. Munro, H.N., and Fleck, A. (1966) inMethods of Biochemical Analysis (Glick, D., ed.) Vol. 14, pp. 113–176, Interscience Publication, New York.

    Chapter  Google Scholar 

  21. Setaro, F., and Morley, C.D.G. (1977)Anal. Biochem. 81, 467–471.

    Article  PubMed  CAS  Google Scholar 

  22. Almog, R., and Shirley, T.L. (1978)Anal. Biochem. 91, 130–137.

    Article  PubMed  CAS  Google Scholar 

  23. Warren, L. (1959)J. Biol. Chem. 234, 1971–1975.

    PubMed  CAS  Google Scholar 

  24. Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  25. Börgstorm, B. (1952)Acta Physiol. Scand. 25, 101–110.

    Article  Google Scholar 

  26. Wood, R., and Falch, J. (1974)Lipids 9, 979–986.

    Article  PubMed  CAS  Google Scholar 

  27. Wood, R., and Lee, T. (1981)J. Biol. Chem. 256, 12379–12386.

    PubMed  CAS  Google Scholar 

  28. Gerlach, U., and Hiby, W. (1974) inMethods of Enzymatic Analysis (Bergmeyer, H.U., ed.) Vol. 2, pp. 871–875, Academic Press, New York.

    Google Scholar 

  29. Nakao, K., Kurashina, S., and Nakao, M. (1967)Life Sciences 6, 595–600.

    Article  PubMed  CAS  Google Scholar 

  30. Kilberg, M.S., and Christensen, H.N. (1979)Biochemistry 18, 1525–1530.

    Article  PubMed  CAS  Google Scholar 

  31. Stocco, D.M., and Hutson, J.C. (1980)Cancer Res. 40, 1486–1492.

    PubMed  CAS  Google Scholar 

  32. Leroy-Houyet, M., Quintart, J., and Baudhuin, P.J. (1979)Ultrastructures Res. 69, 68–85.

    Article  CAS  Google Scholar 

  33. Fiala, S., and Fiala, A.E. (1967)Intl. J. Cancer 2, 344–354.

    CAS  Google Scholar 

  34. Emmelot, P., and Box, C.J. (1972)J. Membrane Biol. 9, 83–104.

    Article  CAS  Google Scholar 

  35. Shimizu, S., and Funakoshi, I. (1970)Biochim. Biophys. Acta 203, 167–169.

    Article  PubMed  CAS  Google Scholar 

  36. Dnistrian, A.M., Skipski, V.P., Barclay, M., and Stock, C.C. (1977)Cancer Res. 37, 2182–2187.

    PubMed  CAS  Google Scholar 

  37. Colbeau, A., Nachbaur, J., and Vignais, P.M. (1971)Biochim. Biophys. Acta 249, 462–492.

    Article  PubMed  CAS  Google Scholar 

  38. Pessino, V., Vigneri, R., Siperstein, M.D., and Goldfine, I.D. (1979)Cancer Res. 39, 1443–1446.

    Google Scholar 

  39. Merisko, E.M., Ojakian, G.K., and Widnell, C.C., (1981)J. Biol. Chem. 256, 1983–1993.

    PubMed  CAS  Google Scholar 

  40. Stanley, K.K., and Paul-Luzio, J. (1978)Biochim. Biophys. Acta 514, 198–205.

    Article  PubMed  CAS  Google Scholar 

  41. Bergeron, J.J.M. (1975)Biochim. Biophys. Acta 407, 325–337.

    PubMed  CAS  Google Scholar 

  42. Siperstein, M.D., and Fagan, V.M. (1964)Cancer Res. 24, 1108–1115.

    PubMed  CAS  Google Scholar 

  43. Sabine, J.R., Abraham, S., and Chaikoff, I.L. (1967)Cancer Res. 27, 793–799.

    PubMed  CAS  Google Scholar 

  44. Figard, P.H., and Greenberg, D.M. (1962)Cancer Res. 22, 361–367.

    PubMed  CAS  Google Scholar 

  45. Dennis, E.A., and Kennedy, E.P. (1972)J. Lipid Res. 13, 263–267

    PubMed  CAS  Google Scholar 

  46. Patton, S. (1970)J. Theor. Biol. 29, 489–491.

    Article  PubMed  CAS  Google Scholar 

  47. Vandenheuvel, F.A. (1963)J. Am. Oil Chem. Soc. 40, 455–471.

    Google Scholar 

  48. Demel, R.A., Jansen, J.W.C.M., Van Dijck, P.W.M., and Van-Deenen, L.L.M. (1977)Biochim. Biophys. Acta 465, 1–10.

    Article  PubMed  CAS  Google Scholar 

  49. Estep, T.N., Mountcastel, D.B., Barenholz, Y., Biltonen, R.L., and Thompson, T.E. (1979)Biochemistry 18, 2112–2117.

    Article  PubMed  CAS  Google Scholar 

  50. Shinitzky, M., and Barenholz, Y. (1974)J. Biol. Chem. 249, 2652–2657.

    PubMed  CAS  Google Scholar 

  51. Kirk, G.A. (1977)Biochim. Biophys. Acta 464, 157–164.

    Article  PubMed  CAS  Google Scholar 

  52. Borochov, H., Zahler, P., Wilbrandt, W., and Shinitzky, M. (1977)Biochim. Biophys. Acta 470, 382–388.

    Article  CAS  Google Scholar 

  53. DiCorleto, P.E., Warach, J.B., and Silversmit, D.B. (1979)J. Biol. Chem. 254, 7795–7802.

    PubMed  CAS  Google Scholar 

  54. Verkleij, A.J., Zwaal, R.F.A., Roelofsen, B., Comfurius, P., Kastelijn, D., and VanDeenen, L.L.M. (1973)Biochim. Biophys. Acta 323, 178–193.

    Article  PubMed  CAS  Google Scholar 

  55. Singer, S.J., and Nicholson, G.L. (1972)Science 175, 720–731.

    Article  PubMed  CAS  Google Scholar 

  56. McMurray, W.C. (1973) inForm and Function of Phospholipids (Ansell, G.B., Hawthorne, J.N., and Dawson, R.M.C., eds.) Vol. 3, Elsevier Scientific Publishing Co., New York.

    Google Scholar 

  57. Wood, R. (1974)Lipids 8, 830–832.

    Article  Google Scholar 

  58. Wood, R., and Falch, J. (1973)Lipids 8, 702–710.

    Article  PubMed  CAS  Google Scholar 

  59. Emmelot, P. (1977) inMammalian Cell Membranes (Jamieson, G.A., and Robinson, D.M., eds.) Vol. 2, pp. 1–54, Butterworths, London.

    Google Scholar 

  60. Rothman, J.E., and Lenard, J. (1979)Science 197, 743–753.

    Google Scholar 

  61. Dennis, E.A., and Kennedy, E.P. (1972)J. Lipids Res. 13, 263–267.

    CAS  Google Scholar 

  62. Wood, R., and Harlow, R.D. (1969)Arch. Biochem. Biophys. 135, 272–281.

    Article  PubMed  CAS  Google Scholar 

  63. Wood, R., and Chumbler, F. (1978)Lipids 13, 75–84.

    Article  PubMed  CAS  Google Scholar 

  64. Wood, R., and Harlow, R.D. (1969)Arch. Biochem. Biophys. 131, 495–501.

    Article  PubMed  CAS  Google Scholar 

  65. Wood, R. (1975)Lipids 10, 736–745.

    Article  PubMed  CAS  Google Scholar 

  66. Berde, C.B., Anderson, H.C., and Hudson, B.S. (1980)Biochemistry 19, 4279–4293.

    Article  PubMed  CAS  Google Scholar 

  67. Cook, S.L., Bouma, S.R., and Huestis, W.H. (1980)Biochemistry 19, 4601–4607.

    Article  PubMed  CAS  Google Scholar 

  68. Briggs, M.M., and Lefkowitz, M.M. (1980)Biochemistry 19, 4461–4466.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Texas Agricultural Experiment Station, Texas A&M University System, 77843, College Station, TX

    Randall Wood, Girish C. Upreti & Roberto J. deAntueno

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  1. Randall Wood
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  2. Girish C. Upreti
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  3. Roberto J. deAntueno
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Wood, R., Upreti, G.C. & deAntueno, R.J. A comparison of lipids from liver and hepatoma subcellular membranes. Lipids 21, 292–300 (1986). https://doi.org/10.1007/BF02536416

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  • DOI: https://doi.org/10.1007/BF02536416

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