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In vitro conversion of erucic acid by microsomes and mitochondria from liver, kidneys and heart of rats

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Lipids

Abstract

Microsomes and mitochondria of liver, kidneys, and heart were incubated with [14-14C]erucic acid in three assay media: one favorable for chain elongation (NADPH+KCN), another favorable for β-oxidation and the last one for shortening (NADP+KCN). Elongating reactions occurred mainly in microsomes, those of kidneys being very active; the mitochondria also showed some activity, heart mitochondria being, however, more active than the microsomes, when considering the amount of erucic acid activated. In the medium for β-oxidation, practically no shortened fatty acids were found. On the contrary, when β-oxidation was inhibited, and in the presence of NADP, the formation of shorter monoenes, probably in the outer membrane of the mitochondria, was observed, namely eicosenoic acid in high amount, oleic acid and hexadecenoic acid. Mitochondria from liver were very active as were those of heart, when compared with the quantity of activated erucic acid. In heart, the mitochondria shortened erucic acid into oleic acid and hexadecenoic acid, which were then probably used as energy substrates. With carnitine and without NADP, shortened fatty acids were formed in the mitochondria of liver, probably by the first reactions of β-oxidation. In this case, the proportions of oleic acid and hexadecenoic acid were higher than with NADP alone. In the presence of carnitine and NADP, the level of the chain-shortening reaction did not differ from that observed with NADP alone. It appears, therefore, that the activated erucic acid is mainly directed towards shortening reactions and not towards transfer reactions across the mitochondrial membranes.

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References

  1. Roine, P., E. Uksila, H. Teir, and J. Rapolar, Z. Ernaehrungswiss. 1: 118 (1960).

    Article  CAS  Google Scholar 

  2. Abdellatif, A.M.M., and R.O. Vles, Nutr. Metab. 12:285 (1970).

    Article  PubMed  CAS  Google Scholar 

  3. Rocquelin, G., R. Cluzan, R. Levillain, N. Vodovar, and J. Causeret, Arch. Mal. Coeur 66: 1085 (1973).

    PubMed  CAS  Google Scholar 

  4. Beare-Rogers, J.L., E.A. Nera, and H.A. Heggtveit, Nutr. Metab. 17:213 (1974).

    PubMed  CAS  Google Scholar 

  5. Vles, R.O., in “The Role of Fats in Human Nutrition,” Edited by A.J. Vergroessen, Academic Press, London, 1975, p. 434.

    Google Scholar 

  6. Creisteil, T., P. Ketevi, and D. Lapous, C.R. Acad. Sci. Paris 275:1443 (1972).

    Google Scholar 

  7. Dallochio, M., J. Larrue, M. Rabaud, G. Razaka, R. Crockett, and H. Bricaud, C.R. Soc. Biol. Paris 167:257 (1973).

    Google Scholar 

  8. Collomb, M.H., R. Albrecht, G. Griffaton, P. Manchon, and R. Lowy, Enzyme 18:300 (1974).

    PubMed  CAS  Google Scholar 

  9. Gaillard, D., B. Pipy, and R. Derache, Toxicology 2:165 (1974).

    Article  PubMed  CAS  Google Scholar 

  10. Carroll, K.K., Can. J. Biochem. Physiol. 40: 1229 (1962).

    PubMed  CAS  Google Scholar 

  11. Bach, A., P. Métais, J. Raulin, and R. Jacquot, Bull. Soc. Chim. Biol. 51:167 (1969).

    PubMed  CAS  Google Scholar 

  12. Craig, B.M., and J.L. Beare, Can. J. Biochem. Physiol. 45: 1075 (1967).

    CAS  Google Scholar 

  13. Carreau, J.P., A. Thoron, D. Lapous, and J. Raulin, Bull. Soc. Chim. Biol. 50:1973 (1968).

    PubMed  CAS  Google Scholar 

  14. Ong, N., J. Bézard, and J. Lecerf, Lipids 12:563 (1967).

    Article  Google Scholar 

  15. Carroll, K.K., Lipids 1: 171 (1966).

    Article  CAS  PubMed  Google Scholar 

  16. Boucrot, P., and J. Bézard, Arch. Sci. Physiol. 27:1 (1973).

    CAS  Google Scholar 

  17. Lecerf, J., and J. Bézard, Biochimie 56:599 (1974).

    PubMed  CAS  Google Scholar 

  18. Vasdev, S.C., and K.J. Kako, Biochim. Biophys. Acta 431:22 (1976).

    PubMed  CAS  Google Scholar 

  19. Pinson, A., and P. Padieu, FEBS Lett. 39:88 (1974).

    Article  PubMed  CAS  Google Scholar 

  20. Hubbell, R.B., L.B. Mendel, and A.J. Wakeman, J. Nutr. 14:273 (1937).

    CAS  Google Scholar 

  21. Layne, E., in “Methods in Enzymology,” Vol. III, Edited by Colowick and Kaplan, Academic Press, New York, 1957, p. 447.

    Google Scholar 

  22. Clouet, P., H. Carlier, and A. Chaibi, C.R. Acad. Sci. Paris 277:1177 (1973).

    CAS  Google Scholar 

  23. Clouet, P., H. Carlier, J.P. Blond, and J. Bézard, Ann. Nutr. Alim. 30:537 (1976).

    CAS  Google Scholar 

  24. Blond, J.P., P. Clouet, and P. Lemarchal, Biochemie 57:361 (1975).

    CAS  Google Scholar 

  25. Mohrhauer, H., K. Christiansen, M.V. Gan, M. Deubig, and R.T. Holman, J. Biol. Chem. 242:4507 (1967).

    PubMed  CAS  Google Scholar 

  26. Sprecher, H., Biochim. Biophys. Acta 360: 113 (1974).

    PubMed  CAS  Google Scholar 

  27. Bremer, J., J. Biol. Chem. 238:2774 (1963).

    PubMed  CAS  Google Scholar 

  28. Nugteren, D.H., Biochim. Biophys. Acta 106: 280 (1965).

    PubMed  CAS  Google Scholar 

  29. Bézard, J., P. Boucrot, and G. Clément, J. Chromatogr. 14:368 (1964).

    Article  Google Scholar 

  30. Seubert, W., and E.R. Podack, Mol. Cell. Biochem. 1:29 (1973).

    Article  PubMed  CAS  Google Scholar 

  31. De Jong, J.W., and W.C. Hülsmann, Biochim. Biophys. Acta 197:127 (1970).

    Article  PubMed  Google Scholar 

  32. Stanley, K.K., and P.K. Tubbs, Biochem. J. 150:77 (1975).

    PubMed  CAS  Google Scholar 

  33. Van Tol, A., and W.C. Hülsmann, Biochim. Biophys. Acta 189:342 (1969).

    Article  PubMed  Google Scholar 

  34. Hoppel, C.L., and R.J. Tomec, J. Biol. Chem. 247:832 (1972).

    PubMed  CAS  Google Scholar 

  35. Chase, J.F.A., and P.K. Tubbs, Biochem. J. 129:55 (1972).

    PubMed  CAS  Google Scholar 

  36. Swarttouw, M.A., Biochim. Biophys. Acta 337:13 (1974).

    PubMed  CAS  Google Scholar 

  37. Beare-Rogers, J.L., E.A. Nera, and B.M. Craig, Lipids 7:548 (1972).

    Article  PubMed  CAS  Google Scholar 

  38. Houtsmuller, U.M.T., C.B. Struijk, and A.v.d. Beek, Biochim. Biophys. Acta 218:546 (1970).

    Google Scholar 

  39. Rocquelin, G., R. Cluzan, N. Vodovar, and R. Levillain, Cah. Nutr. Diet. 8:103 (1973).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Physiologie animale et de la Nutrition, Faculté des Sciences Mirande, B.P. 138, 21004, DIJON Cédex, France

    Pierre Clouet & Jean Bezard

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  1. Pierre Clouet
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  2. Jean Bezard
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Clouet, P., Bezard, J. In vitro conversion of erucic acid by microsomes and mitochondria from liver, kidneys and heart of rats. Lipids 14, 268–273 (1979). https://doi.org/10.1007/BF02533913

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  • DOI: https://doi.org/10.1007/BF02533913

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