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Molecular cloning and characterization of small and large subunits of ADP-glucose pyrophosphorylase from oriental melon

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Abstract

We isolated three cDNAs encoding one small subunit and two large subunits of ADP-glucose pyrophosphorylase (AGP) from oriental melon.msf1 encoding the small subunit was 2,027 bp in length, andmlf1 andmlf2 encoding the large subunits were 2,009 and 2,227 bp, respectively. Each of the three clones had a long, uninterrupted open reading frame, deriving 57–58 kDa polypeptide. The strongest expression ofmsf1 occurred in leaves, and a relatively weak expression in roots and stems.mlf1 was highly expressed in stems and roots, whereas the expression was much weaker in leaves. The expression pattern ofmlf2 was similar to that ofmsf1, but the expression level ofmlf2 transcripts was much lower than that ofmsf1 transcripts. In fruits,msf1, mlf1, andmlf2 were expressed in all stages investigated with a similar expression profile. During fruit development, all three genes showed the highest expression at the early stage, and the transcripts decreased as the fruits matured.

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Abbreviations

AGP:

ADP-glucose pyrophosphorylase

DAP:

days after pollination

3-PGA:

3-phosphoglycerate

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yosong-ku, 305-701, Taejon, Korea

    Seong-Whan Park, Hyung-Yeel Kahng, In-Jung Kim, Jeom-Ok Park & Won-II Chung

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  1. Seong-Whan Park
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  2. Hyung-Yeel Kahng
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  3. In-Jung Kim
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  4. Jeom-Ok Park
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  5. Won-II Chung
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Corresponding author

Correspondence to Won-II Chung.

Additional information

The nucleotides sequence data reported will appear in the EMBL, GenBank, and DDBJ Nucleotide Sequence Databases under the accession numbers AF030382 (msf1), AF030383 (mlf1), and AF030384 (mlf2).

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Park, SW., Kahng, HY., Kim, IJ. et al. Molecular cloning and characterization of small and large subunits of ADP-glucose pyrophosphorylase from oriental melon. J. Plant Res. 111, 59–63 (1998). https://doi.org/10.1007/BF02507150

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  • DOI: https://doi.org/10.1007/BF02507150

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