Skip to main content
SpringerLink
Log in

Unidirectional migration of osteosarcoma cells with osteoblast characteristics in response to products of bone resorption

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

To investigate the mechanisms by which bone-forming cells are attracted to areas of bone resorption during bone remodeling, we have usedin vitro methods to look for signals released by resorbing bone, which may be chemotactic for cultured bone cells. We have found that cultured rat osteosarcoma cells, which have characteristics associated with the osteoblastic phenotype, migrate in a unidirectional manner in response to a signal released by resorbing bones. These cells also migrated unidirectionally in response to Type I collagen, which comprises 95% of the bone matrix. This phenomenon of chemotaxis of bone-forming cells to sites of previous resorption may be an important component of the process of bone remodeling and the coupling of bone formation to bone resorption.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Frost HM. (1967) The dynamics of human osteoid tissue. In: Hioco DJ (ed) L'Osteomalacie” Masson, Paris

    Google Scholar 

  2. Rasmussen H, Bordier P (1974) Bone cells—morphology and physiology. In: The Physiological and Cellular Basis of Metabolic Bone Disease, Williams and Wilkins Co., Baltimore, pp 9–69

    Google Scholar 

  3. Postlethwaite AE, Seyer JM, Kang AH (1978) Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagen-derived peptides. Proc Natl Acad Sci USA 75:871–875

    CAS  PubMed  Google Scholar 

  4. Majeska RJ, Rodan SB, Rodan GA (1978) Maintenance of parathyroid hormone response in clonal rat osteosarcoma lines. Exp Cell Res 111:465–468

    Article  CAS  PubMed  Google Scholar 

  5. Majeska RJ, Rodan SB, Rodan GA (1980) Parathyroid hormone responsive clonal cell lines from rat osteosarcoma. Endocrinology 107:1494

    Article  CAS  PubMed  Google Scholar 

  6. Martin TJ, Ingleton PM, Underwood JCE, Michelangeli VP, Hunt NH, Melick RA (1976) Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260:436–438

    Article  CAS  PubMed  Google Scholar 

  7. Martin TJ, Ingleton PM, Caulton LA, Melick RA (1979) Metabolic properties of hormonally responsive osteogenic sarcoma cell. Clin Orthoped Rel Res 140:247

    CAS  Google Scholar 

  8. Partridge NC, Frampton RJ, Eisman JA, Michelangeli VP, Elms E, Bradley TR, Martin TJ (1980) Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett 115:139–142

    Article  CAS  PubMed  Google Scholar 

  9. Mundy GR, Varani J, Orr W, Gondek MD, Ward PA (1978) Resorbing bone is chemotactic for monocytes. Nature (London) 275:132–136

    Article  CAS  PubMed  Google Scholar 

  10. Orr W, Varani J, Gondek MD, Ward PA, Mundy GR (1979) Chemotactic responses of tumor cells to products of resorbing bone. Science 203:176–179

    CAS  PubMed  Google Scholar 

  11. Trummel CL, Mundy GR, Raisz LG (1975) Release of osteoclast activating factor by normal human peripheral blood leukocytes. J Lab Clin Med 85:1001–1007

    CAS  PubMed  Google Scholar 

  12. Yoneda T, Mundy GR (1979) Monocytes regulate osteoclast activating factor production by releasing prostaglandins. J Exp Med 150:338–350

    Article  CAS  PubMed  Google Scholar 

  13. Schiffman E, Corcoran BA, Aswanikumar S (1978) Molecular events in the response of neutrophils to synthetic Nf Met chemotactic peptides: Demonstration of a specific receptor. In: Gallin JI, Quie PG (eds) Leukocyte Chemotaxis Raven Press, New York, pp 97–111

    Google Scholar 

  14. Rodan GA, Majeska RJ, Rodan SB, Egan JJ (1981) The use of osteosarcoma clonal variants in the study of PTH effects on osteoblast-like cells. In: Cohn DV, Talmage RV, Matthews JL, (eds) Hormonal Control of Calcium Metabolism, Excerpta Medica, Amsterdam p 191

    Google Scholar 

  15. Raisz LG, Canalis EM, Dietrich JW, Kream BE, Gworek SC (1978) Hormonal regulation of bone resorption. In: Recent Progress in Hormone Research, Vol 34, p 335–356

  16. Orr FW, Varani J, Gondek MD, Ward PA, Mundy GR (1980) Partial characterization of a bone derived chemotactic factor for tumor cells, Am J Pathol 99:43–52

    CAS  PubMed  Google Scholar 

  17. Mundy GR, DeMartino S, Rowe DW (1981) Collagen and collagen fragments are chemotactic for tumor cells. J Clin Invest 68:19–22

    Article  Google Scholar 

  18. Howard GA, Bottemiller BL, Baylink DJ (1980) Evidence for the coupling of bone formation to bone resorptionin vitro, Metab Bone Dis Rel Res 2:131–135

    Article  Google Scholar 

  19. Howard GA, Bottemiller BL, Turner RT, Rader JI, Baylink DJ (1981) Parathyroid hormone stimulates bone formation and resorption in organ culture: evidence for a coupling mechanism. Proc Natl Acad Sci USA 78:3204–3208

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Endocrinology and Metabolism, Department of Medicine, University of Texas Health Science Center, 78284, San Antonio, Texas, USA

    Gregory R. Mundy & Carol Trimmier

  2. University of Connecticut Schools of Medicine and Dental Medicine, Farmington, Connecticut, USA

    Sevgi B. Rodan, Robert J. Majeska, Sandra DeMartino & Gideon A. Rodan

  3. Department of Medicine, Repatriation General Hospital, University of Melbourne, West Heidelberg, Victoria, Australia

    T. John Martin

Authors
  1. Gregory R. Mundy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sevgi B. Rodan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert J. Majeska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sandra DeMartino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carol Trimmier
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. John Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gideon A. Rodan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mundy, G.R., Rodan, S.B., Majeska, R.J. et al. Unidirectional migration of osteosarcoma cells with osteoblast characteristics in response to products of bone resorption. Calcif Tissue Int 34, 542–546 (1982). https://doi.org/10.1007/BF02411301

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02411301

Key words

Search

Navigation