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Long-term culture of cells from bone affected by Paget’s disease

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Summary

Cells obtained from surgical bone specimens of eight patients with Paget’s disease of bone were maintained in culture for up to 8 months and seven passages. The doubling time during the period of maximal cell growth ranged from 4 to 12 days. Evidence consistent with the hypothesis that many of the cells were bone cells included the following: (a) histochemical techniques demonstrated staining of some cells for alkaline phosphatase or acid phosphatase and succinic dehydrogenase; (b) parathyroid extract stimulated increased uptake of3H-thymidine and3H-uridine; (c) parathyroid extract suppressed and salmon calcitonin stimulated uptake of3H-proline; and (d) crystalline calcium deposits were found within cells and extracellularly.

Ultrastructural analysis revealed that three of the eight cultures contained cells whose nuclei had inclusions which were almost identical to those found in the osteoclast nuclei of all patients with Paget’s disease. The maintenance of cells derived from pagetic bone in long-term culture should aid in testing the hypothesis that Paget’s disease represents a slow virus infection of bone.

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References

  1. Rebel, A., Malkani, K., Basle, M.: Anomalies nucleaires des osteoclasts de la maladie osseuse de Paget, Nouv. Presse Med.3:1299–1301, 1974

    PubMed  CAS  Google Scholar 

  2. Baldet, P.: La Maladie Osseuse de Paget. Premier Collique de Pathologie Locomotrice, p. 73. Paris Galliena, Montpellier, 1975

    Google Scholar 

  3. Mills, B.G., Singer, F.R.: Nuclear inclusions in Paget’s disease of bone, Science194:201–202, 1976

    PubMed  CAS  Google Scholar 

  4. Schultz A., Delling, G., Ringe, J.-D., Ziegler, R.: Untersuchungen zur ultrastruktur der osteoclasten und ihrer cytopathogenese, Virchows Arch. [Pathol. Anat.]376:309–328, 1977

    Article  Google Scholar 

  5. Oyanagi, S., ter Meulen, V., Katz, M., Koprowski, H.: Comparison of subacute sclerosing panencephalitis and measles viruses: an electron microscope study, J. Virol.7:176–187, 1971

    PubMed  CAS  Google Scholar 

  6. Horta-Barbosa, L., Fuccillo, D.A., Sever, J.L., Zeman, W.: Subacute sclerosing panencephalitis: isolation of measles virus from a brain biopsy, Nature221:974–975, 1969

    PubMed  CAS  Google Scholar 

  7. Merchant, D.J., Kahn, R.H., Murphy, W.H.,Jr.: Handbook of Cell and Organ Culture, pp. 207–209. Burgess Publ. Co., Minneapolis, 1964

    Google Scholar 

  8. Mills, B.G., Holst, P., Haroutinian, A.M., Bavetta, L.A.: Bone cell response to serum calcium altering drugs, Clin. Orthop.78:56–82, 1971

    PubMed  CAS  Google Scholar 

  9. Humason, G.L.: Animal Tissue Techniques, pp. 261–263. W.H. Freeman & Co., San Francisco, 1967

    Google Scholar 

  10. Smith, D.M., Johnston, C.C.,Jr., Severson, A.R.: Studies of the metabolism of separated bone cells, Calcif. Tissue Res11:56–69, 1973

    PubMed  CAS  Google Scholar 

  11. Walker, D.G.: Enzymatic and electron microscopic analysis of isolated osteoclasts, Calcif. Tissue Res.9:296–309, 1972

    Article  PubMed  CAS  Google Scholar 

  12. Fullmer, H.M., Link, C.C.,Jr., Baer, M.J.: A stain for bone illustrating apposition and absorption in two colors, Stain Technol.39:71–73, 1964

    PubMed  CAS  Google Scholar 

  13. Sigma Technical Bulletin No. 85: The histochemical demonstration of phosphatase in blood smears or tissue sections, p. 1. Sigma Chemical Co., St. Louis, 1963

  14. Sigma Technical Bulletin No. 840: The histochemical demonstration of nitroblue tetrazolium (NBT) reduction in neutrophils, pp. 1–15. Sigma Chemical Co., St. Louis, 1978

  15. Humason, G.L.: Animal Tissue Techniques, pp. 416–417. W.H. Freeman & Co., San Francisco, 1967

    Google Scholar 

  16. Dayer, J.-M., Krane, S.M., Russell, R.G.G., Robinson, D.R.: Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells, Proc. Natl. Acad. Sci. U.S.A.73:945–949, 1976

    PubMed  CAS  Google Scholar 

  17. Martin, G.R., Mecca, C.E., Schiffmann, E., Goldhaber, P.: Alterations in bone metabolism induced by parathyroid extract. In P.J. Gaillard, R.V. Talmage, A.M. Budy (eds.): The Parathyroid Glands, pp. 261–272. University of Chicago Press, Chicago, 1965

    Google Scholar 

  18. Peck, W.A., Messinger, K., Kimmich, G., Carpenter, J.: Stimulation of uridine incorporation in isolated bone cells by parathyroid hormone and cyclic AMP, J. Endocrinol.95:289–298, 1974

    CAS  Google Scholar 

  19. Bingham, P.J., Brazell, I.A., Owen, M.A.: The effect of parathyroid extract on cellular activity and plasma calcium levelsin vivo, J. Endocrinol45:387–400, 1969

    Article  PubMed  CAS  Google Scholar 

  20. Rechler, M.M., Podskalny, J.M., Goldfine, I.D., Wells, C.A.: DNA synthesis in human fibroblasts: stimulation by insulin and by non-suppressible insulin-like activity (NSILA-S), J. Clin. Endocrinol. Metab.39:512–521, 1974

    PubMed  CAS  Google Scholar 

  21. Tamm, I., Hand, R., Caliguiri, L.A.: Action of dichlorobenzimidazole riboside on RNA synthesis in L-929 and HeLa cells, J. Cell Biol.69:229–240, 1976

    Article  PubMed  CAS  Google Scholar 

  22. Raisz, L.G., Dietrich, J.W., Canalis, E.M.: Factors influencing bone formation in organ culture, Isr. J. Med. Sci.12:108–114, 1976

    PubMed  CAS  Google Scholar 

  23. Miller, S.S., Wolf, A.M., Arnaud, C.D.: Bone cells in culture: Morphologic transformation by hormones, Science192:1340–1342, 1976

    PubMed  CAS  Google Scholar 

  24. Yagiela, J.A., Woodbury, D.M.: Enzymatic isolation of osteoblasts from fetal calvaria, Anat. Rec.188:287–305, 1977

    Article  PubMed  CAS  Google Scholar 

  25. Binderman, I., Duksin, D., Harell, A., Katzir, E., Sachs, L.: Formation of bone tissue in culture from isolated bone cells, J. Cell Biol.61:427–439, 1974

    Article  PubMed  CAS  Google Scholar 

  26. Luben, R.A., Wong, G.L., Cohn, D.V.: Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts, Endocrinology99:526–534, 1976

    Article  PubMed  CAS  Google Scholar 

  27. Osdoby, P., Caplan, A.I.: The possible differentiation of osteogenic elements in vitro from chick limb mesenchymal cells, Dev. Biol.52:283–299, 1976

    Article  PubMed  CAS  Google Scholar 

  28. Rose, G.G., Shindler, T.O.: The mineralization of emigrating bone cells in tissue culture, Tex. Rep. Biol. Med.22:174–188, 1964

    PubMed  CAS  Google Scholar 

  29. Bard, D.R., Dickens, M.J., Edwards, J., Smith, A.V.: Ultrastructure, in vitro cultivation and metabolism of cells isolated from arthritic human bone, J. Bone Joint Surg.56B:352–360, 1974

    Google Scholar 

  30. Rasmussen, H., Bordier, P.: The Physiological and Cellular Basis of Metabolic Bone Disease, p. 154. Williams & Wilkins Co., Baltimore, 1974

    Google Scholar 

  31. Mills, B.G., Haroutinian, A.M., Holst, P., Bordier, P.J., Tun-Chot, S.: Ultrastructural and cellular changes at the costochondral junction following in vivo treatment with calcitonin or calcium chloride in the rabbit. In Selwyn, Taylor (ed.): Endocrinology 1971, pp. 77–88. William Heinemann Medical Books Ltd., London, 1972

    Google Scholar 

  32. Kather, H., Simon, B.: Adenylate cyclase of human fat cell ghosts, J. Clin. Invest.59:730–733, 1977

    Article  PubMed  CAS  Google Scholar 

  33. Goldring, S.R., Dayer, J.-M., Russell, R.G.G., Mankin, H.J., Krane, S.M.: Response to hormones of cells cultured from human giant cell tumor of bone, J. Clin. Endocrinol. Metab.46:425–433, 1978

    PubMed  CAS  Google Scholar 

  34. Marx, S.J., Aurbach, G.D., Gavin, J.R.,3rd, Buell, D.W.: Calcitonin receptors on cultured human lymphocytes, J. Biol. Chem.249:6812–6816, 1974

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Basic Sciences, School of Dentistry, University of Southern California, University Park, 90007, Los Angeles, California, USA

    B. G. Mills, F. R. Singer, L. P. Weiner & P. A. Holst

  2. Departments of Orthopaedic Surgery, Medicine, Neurology and Microbiology, School of Medicine, University of Southern California, University Park, 90007, Los Angeles, California, USA

    B. G. Mills, F. R. Singer, L. P. Weiner & P. A. Holst

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  1. B. G. Mills
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  2. F. R. Singer
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  3. L. P. Weiner
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  4. P. A. Holst
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Mills, B.G., Singer, F.R., Weiner, L.P. et al. Long-term culture of cells from bone affected by Paget’s disease. Calcif Tissue Int 29, 79–87 (1979). https://doi.org/10.1007/BF02408061

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  • DOI: https://doi.org/10.1007/BF02408061

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