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Regulation of anthocyanin synthesis in carrot suspension cultured cells

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Abstract

From a physiological point of view, a close correlation has been suggested between the induction of embryogenesis, i.e. morphological differentiation, and that of anthocyanln synthesis, i.e. metabolic differentiation. I established a carrot suspension culture system in which anthocyanin synthesis and embryogenesis could be induced in the same culture and studled their relations. The results suggested that induction of anthocyanin and that of cell division, or undifferentiated growth, were the alter natures regulated by 2, 4-dichlorophenoxyacetic acid (2, 4-D). The activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) were found to play key roles in the regulation of anthocyanin synthesis by 2, 4-D. PAL and CHS genes and their transcripts were analyzed in detail, which indicated that there are two PAL genes in carrot, one being transiently and rapidly activated by stress, and the other being specifically induced during the 2, 4-D regulated anthocyanin synthesis.

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Abbreviations

CHS:

chalcone synthase (EC 2.3.1.74): 2, 4-D, 2, 4-dichlorophenoxyacetic acid

−2, 4-D medium:

medium lacking 2, 4-D

+2, 4-D medium:

medium containing 2, 4-D

PAL:

phenylalanine ammonia-lyase (EC. 4.3.1.5)

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  1. Yoshihiro Ozeki

    Present address: Department of Biotechnology. Faculty of Technology, Tokyo University of Agriculture and Technology, Nakamachi 2-24-16, 184, Koganei, Tokyo, Japan

Authors and Affiliations

  1. Department of Biology, College of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, 153, Tokyo, Japan

    Yoshihiro Ozeki

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  1. Yoshihiro Ozeki
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Recipient of the Botanical Society Award of Young Scientists, 1994.

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Ozeki, Y. Regulation of anthocyanin synthesis in carrot suspension cultured cells. J. Plant Res. 109, 343–351 (1996). https://doi.org/10.1007/BF02344483

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