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Regulation of phenylalanine ammonia-lyase genes in carrot suspension cultured cells

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Abstract

Induction of anthocyanin synthesis occurs during metabolic differentiation in carrot suspension cultured cells grown in medium lacking 2,4-dichlorophenoxyacetic acid (2,4-D), and is closely correlated with embryogenesis. Anthocyanin synthesis may also be induced by light-irradiation under different culture conditions. The phenylalanine ammonia-lyase (PAL) gene (TRN-PAL), which was transiently induced by the transfer effect, was also rapidly induced after light-irradiation. However, TRN-PAL was not involved in anthocyanin synthesis. A second PAL gene, ANT-PAL, was involved in anthocyanin synthesis. ANT-PAL was induced during metabolic differentiation in medium lacking 2,4-D parallel with the induction of chalcone synthase (CHS). PAL genes in the carrot genome are expressed differentially depending on the nature of the environmental stimulus, e.g. transfer effect and light, and other parameters which also affect anthocyanin synthesis.

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Abbreviations

CHS:

chalcone synthase

2,4-D:

2,4-dichlorophenoxyacetic acid

GUS:

β-glucuronidase

Luc:

firefly luciferase

PAL:

phenylalanine ammonia-lyase

UV:

ultraviolet

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Authors and Affiliations

  1. Department of Biology, College of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, 153, Tokyo, Japan

    Yoshihiro Ozeki

  2. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    Junko Takeda

Authors
  1. Yoshihiro Ozeki
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  2. Junko Takeda
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Ozeki, Y., Takeda, J. Regulation of phenylalanine ammonia-lyase genes in carrot suspension cultured cells. Plant Cell Tiss Organ Cult 38, 221–225 (1994). https://doi.org/10.1007/BF00033880

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