Summary
A method is described for the preparative HPLC of up to 50mg of carotenoid using a self-packing axially-compressed column. Oxygenated carotenoids, xanthophylls, are separated with silica as the stationary phase and acetone/petroleum ether as the mobile phase, while carotenes are separated using magnesium oxide/polyamide as the stationary phase and undiluted petroleum ether as the mobile phase.
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Isaksen, M., Francis, G.W. Preparative HPLC of carotenoids. Chromatographia 27, 325–327 (1989). https://doi.org/10.1007/BF02321278
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DOI: https://doi.org/10.1007/BF02321278