Abstract
The Carotenoid S is a new C30 bonded silica stationary phase, intended for reversed-phase chromatographic applications, which is more hydrophobic and consequently shows stronger retention in comparison to conventionally used C18 stationary phases. We compared the non-polar selectivities of the columns for homologous alkylbenzenes in acetonitrile—water and methanol–water mobile phases and polar reversed-phase selectivities employing the interaction indices and the Linear Free Energy Relationship models. Further, we investigated possibilities of separations of structurally closely related compounds in the groups of phenolic acids, flavones, phthalic acids and related compounds and of acylglycerols on the new C30 column and with different types of columns for reversed-phase chromatography, including shorter alkyl C4, C8, C18 and phenyl bonded stationary phases. The C30 column has in some aspects properties similar to the non-endcapped Nova-Pak column for separation of some acylglycerols with equal equivalent carbon numbers, but enables separations of longer chain triacylglycerols in a single gradient run.
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This work was supported by the Grant Agency of the Czech Republic (Czech Science Foundation) under project P206/12/0398.
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Published in the topical collection 20th International Symposium on Separation Sciences in Prague with guest editors Aleš Horna and Pavel Jandera.
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Vyňuchalová, K., Jandera, P. Comparison of a C30 Bonded Silica Column and Columns with Shorter Bonded Ligands in Reversed-Phase LC. Chromatographia 78, 861–871 (2015). https://doi.org/10.1007/s10337-015-2899-6
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DOI: https://doi.org/10.1007/s10337-015-2899-6