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Specific Features of the Determination of Xanthophyll Esters under Reversed-Phase HPLC Conditions

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Abstract

Specific features of chromatographic determination of xanthophyll esters are studied using an example of marigold flower lutein diesters under reversed-phase HPLC conditions. The developed two-column method made it possible to establish that, in samples with a low solubility of carotenoids in the used solvent and on a chromatography column in using a mobile phase with a low solubility of carotenoids, the precipitation of diesters is possible, which detrimentally affects the accuracy of the chromatographic determination. A critical factor in this case isì temperature: the storage of samples (solutions) in a refrigerator is not always advisable, because freezing of the main components is possible. It was shown that the use of a mobile phase containing from 0 to 10 vol % acetonitrile in acetone at a temperature not lower than 20°C is acceptable for the separation of all-trans-lutein diesters from cis-derivatives of lutein and zeaxanthin derivatives on “monomeric” C18-phases.

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Authors and Affiliations

  1. Institute of Pharmacy, Chemistry and Biology, Belgorod State National Research University, 308015, Belgorod, Russia

    V. I. Deineka, T. G. Burzhinskaya, I. P. Blinova & L. A. Deineka

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  1. V. I. Deineka
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  2. T. G. Burzhinskaya
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  3. I. P. Blinova
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  4. L. A. Deineka
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Correspondence to V. I. Deineka.

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Translated by V. Kudrinskaya

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Deineka, V.I., Burzhinskaya, T.G., Blinova, I.P. et al. Specific Features of the Determination of Xanthophyll Esters under Reversed-Phase HPLC Conditions. J Anal Chem 78, 759–765 (2023). https://doi.org/10.1134/S1061934823060023

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  • DOI: https://doi.org/10.1134/S1061934823060023

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