Summary
The previously described analytical method for carbohydrates, catecholamines, uric acid, creatine and creatinine using thin-layer chromatography on aminomodified HPTLC plates and subsequent thermal activation of the chromatogram zones is expanded to include several steroid hormones. Specifically, they are the pharmacologically relevant compounds cortisone and hydrocortisone, estradiol and estradiol benzoate, estriol, estrone, methyltestosterone, testosterone and testosterone propionate, prednisolone, pregnandiol and triol, progesterone and Reichstein's S.
This is a preview of subscription content, log in via an institution to check access.
References
H. Jork, W. Funk, W. Fischer, H. Wimmer, “Thin-Layer Chromatography—Reagents and Detection Methods”, VCH, Weinheim/New York/Basel/Cambridge/Tokyo, 1994. Vol. 1b, p. 27 ff.
R. Klaus, W. Fischer, H. E. Hauck, Chromatographia28, 364 (1989).
M. Okamoto, F. Yamada, T. Omori, J. High Resolut. Chromatogr. Chromatogr. Commun.5, 163 (1982).
M. Okamoto, F. Yamada, T. Omori, Chromatographia16, 152 (1982).
R. Klaus, W. Fischer, H. E. Hauck, Chromatographia29, 467 (1990).
R. Klaus, W. Fischer, H. E. Hauck, Chromatographia32, 307 (1991).
R. Klaus, W. Fischer, H. E. Hauck, Chromatographia37, 133 (1993).
Camag Application Note A-58, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klaus, R., Fischer, W. & Hauck, H.E. Analysis and chromatographic separation of some steroid hormones on NH2 layers. Chromatographia 39, 97–102 (1994). https://doi.org/10.1007/BF02320466
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02320466