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Extension of the application of the thermal in-situ reaction on NH2 layers to the detection of catecholamines in biological materials

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This article describes a thin-layer chromatographic method for the determination of catecholamines in human urine. Chromatographic separation on NH2-modified silica gel layers is followed by in-situ visualization brought about by heating the plate. The use of this derivatization technique for other urine components, such as creatine, creatinine, uric acid and glucose, is also discussed.

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Authors and Affiliations

  1. Fichtestraße 25, 64285, Darmstadt, Germany

    R. Klaus

  2. E. Merck, 64271, Darmstadt, Germany

    W. Fischer & H. E. Hauck

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  1. R. Klaus
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  2. W. Fischer
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  3. H. E. Hauck
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Klaus, R., Fischer, W. & Hauck, H.E. Extension of the application of the thermal in-situ reaction on NH2 layers to the detection of catecholamines in biological materials. Chromatographia 37, 133–143 (1993). https://doi.org/10.1007/BF02275850

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  • DOI: https://doi.org/10.1007/BF02275850

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