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Application of a thermalin situ reaction for fluorimetric detection of carbohydrates on NH2-layers

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Summary

Based on experience in the purely thermally induced (no analytical reagents) fluorimetric detection of glucose and fructose after thin-layer chromatographic separation on NH2-layers, the application of this method to other sugars is described. After separation with mobile phases containing pyridine, this “dry”in situ derivatization permits a very simple qualitative and quantitative method of detection for the 20 carbohydrates studied.

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References

  1. R. Klaus, W. Fischer, H. E. Hauck, Chromatographia28, 364 (1989).

    Google Scholar 

  2. I. G. Menzies, J. N. Mount, Med. Lab. Techn.32, 269 (1975).

    Google Scholar 

  3. R. Klaus, J. Ripphahn, J. Chromatogr.244, 99 (1982).

    Google Scholar 

  4. R. Klaus, W. Fischer, Methods in Enzymology, Vol. 160 Part A, p. 159, Academic Press, Inc. San Diego, New York, Berkeley, Boston, London, Sidney, Tokyo, Toronto 1988.

    Google Scholar 

  5. W. Jost, H. E. Hauck, J. Chromatogr.261, 235 (1983).

    Google Scholar 

  6. W. Jost, H. E. Hauck, Anal. Biochem.135, 120 (1983).

    Google Scholar 

  7. H. Tanner, Z. Obst.-Weinbau103, 610 (1967).

    Google Scholar 

  8. R. Klaus, J. Chromatogr.333, 276 (1985).

    Google Scholar 

  9. H.-D. Belitz, W. Grosch: Lehrbuch der Lebensmittelchemie, Springer-Verlag, Berlin, Heidelberg, New York 1982.

    Google Scholar 

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Authors and Affiliations

  1. Fichtestraße 25, D-6100, Darmstadt, FRG

    R. Klaus

  2. E. Merck, V Reag Chrom, Postfach 4119, D-6100, Darmstadt

    W. Fischer

  3. E. Merck, Fo Reag Chrom, Postfach 4119, D-6100, Darmstadt

    H. E. Hauck

Authors
  1. R. Klaus
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  2. W. Fischer
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  3. H. E. Hauck
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Klaus, R., Fischer, W. & Hauck, H.E. Application of a thermalin situ reaction for fluorimetric detection of carbohydrates on NH2-layers. Chromatographia 29, 467–472 (1990). https://doi.org/10.1007/BF02261396

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  • DOI: https://doi.org/10.1007/BF02261396

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