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Comparison of tissue culture and whole plant responses to salinity in potato

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Abstract

Salt sensitivities of six potato cultivars using six levels of sodium chloride (0.0 to 0.25M) were studied in a greenhouse. Responses of these cultivars were also determined in tissue culture by studying rooting of stem segments, increase in length of cultured roots and inhibition of growth of cell suspension cultures using similar salt concentrations. Responses of cultured stem segments and cell suspensions differed from those expressed by whole plants. A close similarity was observed between the salt stress response of whole plants and of cultured roots. The latter technique may provide a preliminary screening method for assessing salt tolerance in potato genotypes.

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Abbreviations

BA:

6-benzyladenine

GA3 :

gibberellic acid

I50 :

concentration which inhibits growth 50%

MS:

Murashige and Skoog (1962) medium

NAA:

naphthaleneacetic acid

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Author notes

  1. Prakash S. Naik

    Present address: Division of Genetics & Plant Breeding, Central Potato Research Institute, 171 001, Shimla, HP, India

Authors and Affiliations

  1. Department of Agronomy, University of Illinois, 61801, Urbana, Illinois, USA

    Prakash S. Naik & Jack M. Widholm

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  1. Prakash S. Naik
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  2. Jack M. Widholm
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Naik, P.S., Widholm, J.M. Comparison of tissue culture and whole plant responses to salinity in potato. Plant Cell Tiss Organ Cult 33, 273–280 (1993). https://doi.org/10.1007/BF02319012

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  • DOI: https://doi.org/10.1007/BF02319012

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