Skip to main content
SpringerLink
Log in

Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation

  • Original Research Papers
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The endogenous levels of isopentenyladenine, isopentenyladenosine, zeatin and zeatin riboside and the ability forin vitro axillary shoot organogenesis and rhizogenesis were compared between mature and rejuvenated clones ofHevea brasiliensis (Müll. Arg.). Enhancement of thein vitro organogenesis ability of rubber-tree clones following somatic embryogenesis or repeated grafting onto juvenile rootstocks was accompanied by an increase of zeatin riboside levels in shoots used as starting material forin vitro micropropagation. Furthermore, the zeatin level, inin vitro shoots of clones treated byin vitro micrografting, and consequently capable of axillary shoot and root organogenesis, was higher than inin vitro shoots of non treated mature material incapable of in vitro organogenesis. We conclude that the endogenous zeatin-like cytokinin level (free and ribosylated forms) can be considered as a reliable marker for the recovery ofin vitro shoot and root organogenesis after rejuvenating treatments in rubber-tree clones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ZR:

zeatin riboside

2iP:

isopentenyladenine

iPA:

isopentenyladenosine

NAA:

α-naphthaleneacetic acid

BA:

6-benzyladenine

IBA:

indole-3-butyric acid

References

  • Carron MP, Enjalric F, Lardet L & Deschamps A (1989) Rubber (Hevea brasiliensis Müll. Arg.). In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 5 (pp 222–245). Springer-Verlag, Berlin

    Google Scholar 

  • Carron MP, Etienne H, Lardet L, Campagna S, Perrin Y, Leconte A & Chaine C (1994) Somatic embryogenesis in Rubber (Hevea brasiliensis Müll. Arg.): expression features yield of the technique, field growth and juvenility of the somaplants. In: Jain SM, Gupta PK & Newton RJ (Eds) Somatic embryogenesis in Woody Plants (pp 117–136). Kluwer Academic, The Netherlands

    Google Scholar 

  • Coleman GD & Ernst SG (1990) Axillary shoot proliferation and growth ofPopulus deltoïdes shoot cultures. Plant Cell Rep. 9: 165–167

    Article  CAS  Google Scholar 

  • Das T & Mitra GC (1990) Micropopagation ofEucalyptus teraticornis Smith.. Plant Cell Tiss. Org. Cult. 22: 95–103

    Article  CAS  Google Scholar 

  • Dumas E, Franclet A & Monteuuis O (1989) Microgreffage de méristèmes primaires caulinaires de pins maritimes (Pinus pinaster Ait.) âgés sur de jeunes semis cultivésin vitro. C. R. Acad. Sci. Ser.2, 309: 723–728

    Google Scholar 

  • Enjalric F & Carron MP (1982) Microbouturage in vitro de jeunes plants d'Hevea brasiliensis (Kunth) Müll. Arg.. C. R. Acad. Sci. Ser. III 295: 259–264

    Google Scholar 

  • Fourret Y, Larrieu C & Arnaud Y (1988) Rajeunissementin vitro chez le Sequoia sempervirens (Endl.). Ann. AFOCEL 1988: 55–82

    Google Scholar 

  • Franclet A (1983) Rajeunissement, culturein vitro et pratique sylvicole. Bull. Soc. bot. Fr. 130: 87–98

    Google Scholar 

  • Galoch E (1985) Comparison of the content of growth regulators in juvenile and adult plants of Birch. Acta Physiol. Plant. 7: 205–215

    CAS  Google Scholar 

  • Haffner V (1991) Recherches de marqueurs de la juvenilité/maturité et de la réactivitéin vitro lors du microbouturage chezHevea brasiliensis; relation entre la juvénilité et la réactivitéin vitro. Ph.D. Thesis, University Paris VI, France, 184 p.

    Google Scholar 

  • Hendry NS, Van Staden J & Allan P (1982) Cytokinins in Citrus. II. Fluctuations during growth in juvenile and adult plant. Sci. Hort. 17: 247–256

    CAS  Google Scholar 

  • Huang LC, Liu S, Huang BL, Murashige T, Mahdi FM & Van Gundy R (1992a) Rejuvenation ofSequoia sempervirens by repeated grafting of shoot tips onto juvenile rootstocksin vitro. Plant Physiol. 98: 166–173

    CAS  Google Scholar 

  • Huang LC, Hsiao CK, Lee SH, Huang BL & Murashige T (1992b) Restoration of vigor and rooting competence in stem tissues of matureCitrus by repeated grafting of their shoot apices onto freshly germinated seedlingsin vitro. In Vitro Cell Dev. Biol. 28: 30–32

    Google Scholar 

  • Imbault N, Tardieu I, Joseph C, Zaerr JB & Bonnet-Masimbert M (1988) Possible role of isopentenyladenine and isopentenyladenosine in flowering ofPseudotsuga menziesii: endogenous variations and exogenous applications. Plant Physiol. Biochem. 26(3): 289–295

    CAS  Google Scholar 

  • Kuiper D, Kuiper PJC, Lambers H, Schuit J & Staal M (1989) Cytokinin concentration in relation to mineral nutrition and benzyladenine treatement inPlantago major ssp. pleiosperma. Physiol. Plant. 75: 511–517

    CAS  Google Scholar 

  • Label P, Imbault N & Villar M (1994) ELISA Quantification and GCMS identification of abscisic acid in stigma, ovary and pedicel of pollinated poplar flowers (Populus nigra L.). Tree Physiol. 14:521–530.

    CAS  PubMed  Google Scholar 

  • Lardet L (1987) Amélioration des conditions du microbouturage de l'Hevea brasiliensis Müll. Arg.. Ph.D. Thesis, University of Sciences et Techniques du Languedoc, Montpellier France, 242 p.

    Google Scholar 

  • Letham DS & Palni LMS (1983) The biosynthesis and metabolism of cytokinins. Ann. Rev. Plant Physiol. 34: 163–197

    CAS  Google Scholar 

  • Maldiney R, Pelèse F, Pilate g, Sotta B, Sossountzof L & MiginiacE (1986) Endogenous levels of abscissic acid, zeatin and zeatinriboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes. Physiol. Plant. 68: 426–430

    CAS  Google Scholar 

  • Monteuuis O (1986) Microgreffage du sequoia géant. Ann. AFOCEL 1986: 39–61

    Google Scholar 

  • Mullins Mg & Srinivasan C (1976) Somatic embryos and plantlets from ancient clone of the grapevine (cv Cabernet-Sauvignon) by apomixisin vitro. J. Exp. Bot. 27: 1022–1030

    Google Scholar 

  • Perrin Y, Lardet L, Enjalric F & Carron MP (1994) Rajeunissement de clones matures d'Hevea brasiliensis (Müll. Arg.) par microgreffagein vitro. Can. J. Plant Sci. 74: 623–630

    Google Scholar 

  • Pliego-Alfaro F & Murashige T (1987) Possible rejuvenation of adult avocado by graftage onto juvenile rootstockin vitro. Hort. Sci. 22:1321–1324

    Google Scholar 

  • Robbins WJ (1957) Physiological aspects of ageing in plants. Am. J. Bot. 44: 289–294

    Google Scholar 

  • Rohr R, Von Aderkas P & Bonga JM (1989) Ultrastructural changes in haploid embryoids ofLarix decidua during early embryogenesis. Am. J. Bot. 76: 1460–1467

    Google Scholar 

  • Rugini E & Caricato G (1995) Somatic embyogenesis and plant recovery from mature tissues of olive cultivars (Olea europea L.) “Canino” and “Moraiolo”. Plant Cell Rep. 14: 257–260

    Article  CAS  Google Scholar 

  • San-Jose MC, Vieitez AM & Ballester A (1990) Clonal propagation of juvenile and adult trees of sessile oak by tissue culture techniques. Sylvae Genet. 39: 50–55

    Google Scholar 

  • Seneviratne P (1991) Micropropagation of juvenile and matureHevea brasiliensis. Ph. D. Thesis, University of Bath, UK, 278 p.

    Google Scholar 

  • Sotta B, Pilate G, Pelèse F, Sabbagh I, Bonnet-Massimbert M & Maldiney R (1987) An avidin-biotin solid phase ELISA for femtomol isopentenyladenine and isopentenyladenosine measurements in HPLC purified plant extracts. Plant Physiol. 84: 571–573

    CAS  Google Scholar 

  • Thomas JC & Katterman FR (1986) Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681–683

    CAS  Google Scholar 

  • Vankora R, Hsiao KC, Bomman CH & Gaudinova A (1991) Effect of synthetic cytokinins on levels of endogenous cytokinins and respiration patterns ofBeta vulgaris cells in suspension. J Plant Growth Regul. 10: 197–199

    Google Scholar 

Download references

Author information

Author notes

  1. Patrick Doumas

    Present address: ENSA-M/INRA/CNRS URA 2133, Biochimie et Physiologie Moléculaire des Plantes, 34060, Montpellier Cedex 1, France

Authors and Affiliations

  1. CIRAD-CP, B.P. 5035, 34032, Montpellier, France

    Yolande Perrin, Ludovic Lardets & Marc-Philippe Carrons

  2. INRA, Station d'Amélioration des Arbres Forestiers, 45160, Ardon, France

    Patrick Doumas

Authors
  1. Yolande Perrin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick Doumas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ludovic Lardets
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marc-Philippe Carrons
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yolande Perrin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perrin, Y., Doumas, P., Lardets, L. et al. Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation. Plant Cell Tiss Organ Cult 47, 239–245 (1997). https://doi.org/10.1007/BF02318978

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02318978

Key words

Search

Navigation