Summary
Capillary zone electrophoresis (CZE) with multi-wavelength detection has been used for the separation of the main alkaloids fromEschscholtzia californica. Optimum separation has been achieved with a fused silica capillary tube and a running electrolyte at pH 2.8 prepared from a 40 mM solution of citric acid and a 80 mM solution of Na2HPO4. The applied voltage was 20 kV and the capillary thermostating temperature was kept constant at 10°C.
Similar content being viewed by others
References
S. R. Rabel, J. F. Stobaugh, Pharmaceutical Research10, 171 (1993).
Z. Deyl, R. Struzinsky, J. Chromatogr.569, 63 (1991).
R. H. Cheney, Quart. J. Crud. Drugs3, 413 (1964).
A. Rolland, J. Fleurentin, M.-C. Lanhers, C. Younos, R. Misslin, F. Mortier, J. M. Pelt, Planta Med.57, 212 (1991).
A. Rolland, J. Fleurentin, F. Mortier, J. M. Pelt, Phytotherapy22, 18 (1987).
D. Guédon, N. Cappelaere, Phytochemical Analysis1, 77 (1990).
S. A. E. Hakim, Br. J. Ophtalmol.38, 193 (1954).
J. Kardos, G. Blasko, M. Simonyl, Arzneim.-Forsch./Drug Res.36, 939 (1986).
L. Üstünes, G. M. Laakeman, B. Gözler, A. J. Vlietinck, A. Özer, A. G. Herman, J. Nat. Prod.445, 258 (1988).
F. F. Vincieri, S. Celli, N. Mulinacci, E. Speroni, Pharmacol. Res. Commun.20, 41 (1988).
H. Wagner, S. Bladt, E. M. Zgainski, “Drogenanalyse”, Springer-Verlag, Berlin, Heidelberg, New York, 1983; p. 57.
J.-P. Rey, J. Levesque, J.-L. Pousset, J. Chromatogr.587, 314 (1991).
S. F. Y. Li, “Capillary Electrophoresis”, Elsevier, London, New York, Tokyo, 1992; p. 202.
C. Schwer, E. Kenndler, Chromatographia30, 546 (1990).
T.-L. Hunag, Chromatographia35, 395 (1993).
H. J. Issaq, G. M. Janini, I. Z. Atamna, G. M. Muschik, J. Liq. Chromatogr.14, 817 (1991).
J. Jorgenson, K. D. Lukacs, Science222, 266 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stuppner, H., Sturm, S., Mulinacci, N. et al. Capillary zone electrophoretic analysis of the main alkaloids fromEschscholtzia californica . Chromatographia 37, 579–583 (1993). https://doi.org/10.1007/BF02274105
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02274105