Abstract
Ethylene production by auxin-dependent pear cells culturedin vitro falls rapidly when they are deprived of 2,4-D. This phenomenon is associated with a decrease in ACC production. Readdition of 2,4-D causes a resumption of ACC production and ethylene synthesis. Ethylene-forming enzyme (EFE) activity, although never limiting, decreases sharply during 2,4-D depletion and rises again upon addition of 2,4-D. This increase in the EFE activity is not a rapid response to 2,4-D, since it requires several hours. Changes in EFE activity follow the same pattern as changes in 2,4-D concentration; the decrease in EFE activity is also concomitant with a decrease in the ability of 2,4-dinitrophenol to inhibit ethylene production. The possible role of auxins in the modulation of EFE activity is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Acaster MA, Kende H (1983) Properties and partial purification of 1-aminocyclopropane-1-carboxylate synthase. Plant Physiol 72:139–145
Adams DO, Wang SY, Lieberman M (1981) Effect of ethylene biosynthesis inhibitors on methionine incorporation into spermine and spermidine in apple suspension cells. Plant Physiol 67:S-50
Balagué C, Latché A, Fallot J, Pech JC (1982) Some physiological changes occurring during the senescence of auxin-deprived pear cells in culture. Plant Physiol 69:1339–1343
Balagué C, Soussountzov L, Petitprez M (1983) Ultrastructural changes during senescence of pear cells during auxin starvation. Cytobios 36:53–63
Bates GW, Goldsmith MHM (1983) Rapid response of the plasma membrane potential in oat coleoptiles to auxin and weak acids. Planta 159:231–237
Bufler G, Mor Y, Reid MS, Yang SF (1980) Changes in 1-aminocyclopropane-1-carboxylic acid of cut carnation flowers on relation to their senescence. Planta 150:439–442
Codron H, Latché A, Pech JC, Nébié B, Fallot J (1979) Control of quiescence and viability in auxin deprived pear cells in batch and continuous culture. Plant Sci Lett 17:23–35
Evensen KE (1984) Calcium effects on ethylene and ethane production and 1-aminocyclopropane-1-carboxylic acid content in potato disks. Physiol Plant 60:125–128
Guy M, Kende H (1984) Conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by isolated vacuoles ofPisum sativum L. Planta 160:281–287
Hoffman NE, Yang SF (1980) Changes of 1-aminocyclopropane-1-carboxylic content in ripening fruits in relation to their ethylene production. J Am Soc Hort Sci 105:492–495
John P (1983) Hypothesis. The coupling of ethylene biosynthesis to a transmembrane electrogenic proton flux. FEBS Lett 152(2):141–143
Jones JF, Kende H (1979) Auxin-induced ethylene biosynthesis in subapical stem sections of etiolated seedlings ofPisum sativum L. Planta 146:649–656
Legge RL, Thomson JE, Baker JE, Lieberman M (1982) The effect of calcium on the fluidity and phase properties of microsomal membranes isolated from postclimacteric golden delicious apples. Plant Cell Physiol 23(2):161–169
Lieberman M (1979) Biosynthesis and action of ethylene. Annu Rev Plant Physiol 30:535–591
Lieberman M, Wang SY (1982) Influence of calcium and magnesium on ethylene production by apple tissue slices. Plant Physiol 69:1150–1155
Lieberman M, Wang SY, Owens LD (1979) Ethylene production by callus and suspension cells from cortex tissue of post-climacteric apples. Plant Physiol 63:811–815
Lizada MCC, Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Ann Biochem 100:140–145
Mackenzie IA, Street HE (1970) Studies on growth in culture of plant cells. I. Production of ethylene by suspension cultures ofAcer pseudoplatanus L. J Exp Bot 21:824–834
Mayak S, Legge RL, Thompson JE (1981) Ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes from senescing carnation flowers. Planta 153:49–55
Meyer Y, Aspart L, Chartier Y (1984) Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplasts cultivatedin vitro. Plant Physiol 75:1027–1033
Mousdale DMA (1981) Reversed-phase ion-pair high performance liquid chromatography of the plant hormones indolyl-3-acetic and absicissic acid. J Chromatogr 209:489–493
Pech JC, Romani R (1979) Senescence of pear fruit cells cultured in a continuously-renewed auxin deprived medium. Plant Physiol 64:814–817
Platt-Aloia JA, Thomson WW (1981) Ultrastructure of the mesocarp of mature avocado fruit and changes associated with ripening. Ann Bot 451–465
Puschmann R, Romani R (1983) Ethylene production by auxin-deprived, suspension-cultured pear fruit cells in response to auxins, stress, or precursor. Plant Physiol 73:1013–1019
Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189
Yoshii H, Imaseki H (1981) Biosynthesis of auxin-induced ethylene, effects of indole-3-acetic acid, benzyladenine and abscissic acid on endogenous levels of 1-aminocyclopropane-1-carboxylic acid (ACC) and ACC synthase. Plant Cell Physiol 22:369–379
Yoshii H, Imaseki H (1982) Regulation of auxin-induced ethylene biosynthesis. Repression of inductive formation of 1-aminocyclopropane-1-carboxylic synthase by ethylene. Plant Cell Physiol 23(4):639–649
Yu YB, Adams DO, Yang SF (1979) 1-Aminocyclopropane-1-carboxylate synthase, a key enzyme in ethylene biosynthesis. Arch Biochem Biophys 198:280–286
Yu YB, Adams DO, Yang SF (1980) Inhibition of ethylene production by 2,4-dinitrophenol and high temperature. Plant Physiol 66:286–290
Zurfluh LL, Guilfoyle TJ (1980) Auxin-induced changes of protein synthesis in soybean hypocotyl. Proc Natl Acad Sci USA 77:357–361
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Balagué, C., Pech, JC. Relationship between the activity of the ethylene-forming enzyme and the level of intracellular 2,4-dichlorophenoxyacetic acid in pear cell culturesin vitro . J Plant Growth Regul 4, 81–89 (1985). https://doi.org/10.1007/BF02266946
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02266946