Abstract
Chromosomes formedde novo which originated from the centromeric region of mouse chromosome 7, have been analysed. These new chromosomes were formed by apparently similar large-scale amplification processes, and are organized into amplicons of ∼30 Mb. Centromeric satellite DNA was found to be the constant component of all amplicons. Satellite DNA sequences either bordered the large euchromatic amplicons (E-type amplification), or made up the bulk of the constitutive heterochromatic amplicons (H-type amplification). Detailed analysis of a heterochromatic megachromosome formedde novo by an H-type amplification revealed that it is composed of a tandem array of 10–12 large (∼30 Mb) amplicons each marked with integrated ‘foreign’ DNA sequences at both ends. Each amplicon is a giant palindrome, consisting of two inverted doublets of ∼7.5-Mb blocks of satellite DNA. Our results indicate that the building units of the pericentric heterochromatin of mouse chromosomes are ∼7.5-Mb blocks of satellite DNA flanked by nonsatellite sequences. We suggest that the formationde novo of various chromosome segments and chromosomes seen in different cell lines may be the result of large-scale E- and H-type amplification initiated in the pericentric region of chromosomes.
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References
Bostock CJ, Christie S (1976) Analysis of t he frequency of sister chromatid exchange in different regions of chromosomes of the Kangaroo rat (Dipodomys ordii).Chromosoma 56: 275–287.
Bostock CJ, Clark EM (1980) Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells.Cell 19: 709–715.
Bullock P, Botchan M (1982) Molecular events in the excision of SV40 DNA from the chromosomes of cultured mammalian cells. In: Schimke RT, ed.Gene Amplification, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, pp 215–224.
Carrano AV, Wolff S (1975) Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac.Chromosoma 53: 361–369.
Chang PS, Cantin EM, Zaia JAet al. (1990) Ribozyme-mediated site-specific cleavage of the HIV-1 genome.Clin Biotech 2: 23–31.
Chen C, Okayama H (1987) High-efficiency transformation of mammalian cells by plasmid DNA.Mol Cell Biol 7: 2745–2752.
Davidson RL, Gerald PS (1976) Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.Somatic Cell Genet 2: 165–176.
Fátyol K, Cserpán I, Praznovszky T, Keresõ J, Hadlaczky Gy (1994) Cloning and molecular characterization of a novel chromosome specific centromere sequence of Chinese hamster.Nucleic Acids Res 22: 3728–3736.
Fechheimer M, Boylan JF, Parker Set al. (1987) Transfection of mammalian cells with plasmid DNA by scrape loading and sonication loading.Proc Natl Acad Sci USA 84: 8463–8467.
Ford M, Fried M (1986) Large inverted duplications are associated with gene amplification.Cell 45: 425–430.
Hadlaczky Gy, Went M, Ringertz N R (1986) Direct evidence for the non-random localization of mammalian chromosomes in the interphase nucleus.Exp Cell Res 167: 1–15.
Hadlaczky Gy, Praznovszky T, Cserpán Iet al. (1991) Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene.Proc Natl Acad Sci USA 88: 8106–8110.
Hilwig I, Gropp A (1973) Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound (‘33258 Hoechst’).Exp Cell Res 81: 474–477.
Holmquist GP, Comings DE (1975) Sister chromatid exchange and chromosome organisation based on a bromodeoxyuridine Giemsa-C-banding technique (TC-banding).Chromosoma 52: 245–259.
Hyrien O, Debatisse M, Buttin G, de Saint Vincent BR (1988) The multicopy appearance of large inverted duplication and the sequence at the inversion joint suggest a new model for gene amplification.EMBO J 7: 407–417.
Looney JE, Ma C, Leu THet al. (1988) The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and remarkably uniform in structure.Mol Cell Biol 8: 5268–5279.
Ma C, Looney JE, Leu TH, Hamlin JL (1988) Organisation and genesis of dihydrofolate reductase amplicons in the genome of a methotrexate-resistant Chinese hamster ovary cell line.Mol Cell Biol 8: 2316–2327.
Ma C, Martin S, Trask B, Hamlin J L (1993) Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells.Genes Dev 7: 605–620.
Morgenstern JP, Land H (1990) Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.Nucleic Acids Res 18: 3587–3596.
Perry P, Wolff S (1974) A new Giemsa method for the differential staining of sister chromatids.Nature 251: 156–158.
Praznovszky T, Keresõ J, Tubak V, Fátyol K, Hadlaczky Gy (1991)De novo chromosome formation in rodent cells.Proc Natl Acad Sci USA 88: 11042–11046.
Sambrook J, Fritsch EF, Maniatis T (1989)Molecular cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
Smith K A, Gorman PA, Stark MB, Groves RP, Stark GR (1990) Distinctive chromosomal structures are formed very early in the amplification of CAD genes in Syrian hamster cells.Cell 63: 1219–1227.
Smith KA, Stark MB, Gorman PA, Stark GR (1992) Fusions near telomeres occur very early in the amplification of CAD genes in Syrian hamster cells.Proc Natl Acad Sci USA 89: 5427–5431.
Stark GR (1993) Regulation and mechanism of mammalian gene amplification.Adv Cancer Res 61: 87–113.
Sugden B. Marsh K, Yates J (1985) A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblast transformed by Epstein-Barr virus.Mol Cell Biol 5: 410–413.
Sumner AT (1972) A simple technique for demonstrating centromeric heterchromatin.Exp Cell Res 75: 304–306.
Sumner A T (1991) Scanning electron microscopy of mammalian chromosomes from prophase to telophase.Chromosoma 100: 410–418.
Toledo F, Le Roscouet D, Buttin G, Debatisse M (1992) Coamplified markers alternate in megabase long inverted repeats and cluster independently in interphase nuclei at early steps of mammalian gene amplification.EMBO J 11: 2665–2673.
Vig BK, Richards BT (1992) Formation of primary constriction and hetrochromatin in mouse does not require minor satellite DNA.Exp Cell Res 201: 292–298.
Wang H C, Fedoroff S (1972) Banding of human chromosomes treated with trypsin.Nature 235: 52–54.
Waring M, Britten R J (1966) Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA.Science 154: 791–794.
Wong AKC, Rattner JB (1988) Sequence organisation and cytological localization of the minor satellite of mouse.Nucleic Acids Res 16: 11645–11661.
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Keresõ, J., Praznovszky, T., Cserpán, I. et al. De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes. Chromosome Res 4, 226–239 (1996). https://doi.org/10.1007/BF02254964
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DOI: https://doi.org/10.1007/BF02254964