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Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac

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Abstract

The frequency of sister chromatid exchanges (SCEs) was determined for the chromosomes (except Y2) of the Indian muntjac stained by the fluorescence plus Giemsa (FPG) or harlequin chromosome technique. The relative DNA content of each of the chromosomes was also measured by scanning cytophotometry. After growth in bromodeoxyuridine (BrdU) for two DNA replication cycles, SCEs were distributed according to the Poisson formula in each of the chromosomes. The frequency of SCE in each of the chromosomes was directly proportional to DNA content. A more detailed analysis of SCEs was performed for the three morphologically distinguishable regions of the X-autosome composite chromosome. The SCE frequency in the euchromatic long arm and short arm were proportional to the amount of DNA. In contrast, the constitutive heterochromatin in the neck of this chromosome contained far fewer SCEs than expected on the basis of the amount of DNA in this region. A high frequency of SCE, however, was observed at the point junctions between the euchromatin and heterochromatin.

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Authors and Affiliations

  1. Biomedical Division, Lawrence Livermore Laboratory, 94550, Livermore, California

    A. V. Carrano

  2. Laboratory of Radiobiology and Department of Anatomy, University of California, 94143, San Francisco, California, USA

    Sheldon Wolff

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  1. A. V. Carrano
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  2. Sheldon Wolff
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Carrano, A.V., Wolff, S. Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjac. Chromosoma 53, 361–369 (1975). https://doi.org/10.1007/BF00294083

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