Skip to main content
SpringerLink
Log in

Viability of embryos following second polar body removal in a mouse model

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Our purpose was to evaluate the effect of the second polar body removal on the viability and developmental potential of the resulting embryos.

Method

The second polar body was removed in 343 mouse oocytes and the developmental potential of the resulting embryos was compared in vitro to that in 223 intact mouse embryos and 222 controls which were placed under the same experimental conditions as those micromanipulated.

Results

The proportion of morphologically normal blastocysts formed by polar body biopsied oocytes (70.8%) was not significantly different from that obtained from the control (81.1%) and intact (85%) oocytes. Cell counts of blastocysts obtained from the manipulated and nonmanipulated oocytes were also not statistically different.

Conclusion

The second polar body biopsy does not have a significant adverse effect on preimplantation development and may be applied for genetic evaluation of oocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Handyside AH, Kontogianni EH, Hardy K, Winston RML: Pregnancies from biopsided human preimplantation embryos sexed by Y-specific DNA amplification. Nature 1990;344:768–770

    Google Scholar 

  2. Dokras A, Sargent IK, Ross C, Gardner RL, Barlow DH: Trophectoderm biopsy in human blastocysts. Hum Reprod 1990;5:821–825

    Google Scholar 

  3. Verlinsky Y, Ginsberg N, Lifchez A, Valle J, Moise J, Strom CM: Analysis of the first polar body: Preconceptual genetic diagnosis. Hum Reprod 1990;5:826–829

    Google Scholar 

  4. Verlinsky Y, Kuliev AM (eds): Preimplantation Diagnosis of Genetic Diseases: A New Technique for Assisted Reproduction. New York, Wiley-Liss, 1993

    Google Scholar 

  5. Verlinsky Y, Cieslak J: Embryological and technical aspects of preimplantation genetic diagnosis.In Preimplantation Diagnosis of Genetic Diseases: A New Technique in Assisted Reproduction, Y Verlinsky, AM Kuliev (eds). New York, Wiley-Liss, 1993, pp 49–67

    Google Scholar 

  6. Dyban A, De Sutter P, Verlinsky Y: Preimplantation cytogenetic analysis.In Preimplantation Diagnosis of Genetic Diseases: A New Technique in Assisted Reproduction, Y Verlinsky, AM Kuliev (eds). New York, Wiley-Liss, 1993, pp 93–127

    Google Scholar 

  7. Statistica: Statsoft (TM), 1994, Vol 1, p 1427

Download references

Author information

Authors and Affiliations

  1. Reproductive Genetics Institute, Chicago, Illinois

    Brian Kaplan, Georg Wolf, Larissa Kovalinskaya & Yury Verlinsky

Authors
  1. Brian Kaplan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Georg Wolf
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Larissa Kovalinskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yury Verlinsky
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaplan, B., Wolf, G., Kovalinskaya, L. et al. Viability of embryos following second polar body removal in a mouse model. J Assist Reprod Genet 12, 747–749 (1995). https://doi.org/10.1007/BF02212905

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02212905

Key words

Search

Navigation