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Genome transfer technique for bovine embryo production using the metaphase plate and polar body

  • Embryo Biology
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Abstract

Despite many studies in humans and mice using genome transfer (GT), there are few reports using this technique in oocytes of wild or domestic animals. Therefore, we aimed to establish a GT technique in bovine oocytes using the metaphase plate (MP) and polar body (PB) as the sources of genetic material. In the first experiment, GT was established using MP (GT-MP), and a sperm concentration of 1 × 106 or 0.5 × 106 spermatozoa/ml gave similar fertilization rates. The cleavage rate (50%) and blastocyst rate (13.6%) in the GT-MP group was lower than that of the in vitro production control group (80.2% and 32.6%, respectively). The second experiment evaluated the same parameters using PB instead of MP; the GT-PB group had lower fertilization (82.3% vs. 96.2%) and blastocyst (7.7% vs. 36.8%) rates than the control group. No differences in the amount of mitochondrial DNA (mtDNA) were observed between groups. Finally, GT-MP was performed using vitrified oocytes (GT-MPV) as a source of genetic material. The cleavage rate of the GT-MPV group (68.4%) was similar to that of the vitrified oocytes (VIT) control group (70.0%) and to that of the control IVP group (81.25%, P < 0.05). The blastocyst rate of GT-MPV (15.7) did not differ neither from the VIT control group (5.0%) nor from the IVP control group (35.7%). The results suggested that the structures reconstructed by the GT-MPV and GT-PB technique develop in embryos even if vitrified oocytes are used.

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Acknowledgements

We thank the Brazilian Agricultural Research Corporation (EMBRAPA) and the Coordination for the Improvement of Higher Education Personnel (CAPES) for financial support, the Qualimax (Luziania GO) and Nippobras (Formosa, GO) slaughterhouses for providing biological material to perform these experiments, and all of the students and workers of EMBRAPA for their support during the course of these experiments.

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Authors and Affiliations

  1. University of Brasilia, DF, Brasília, Brazil

    M. A. N. Dode, F. M. C. Caixeta & A. A. G. Fidelis

  2. Federal University of Espírito Santo, Alegre, ES, Brazil

    L. O. Leme

  3. Federal University of Uberlândia, Uberlândia, MG, Brazil

    L. N. Vargas, T. S. Kawamoto & M. M. Franco

  4. Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil

    M. A. N. Dode & M. M. Franco

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  1. M. A. N. Dode
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Dode, M.A.N., Caixeta, F.M.C., Vargas, L.N. et al. Genome transfer technique for bovine embryo production using the metaphase plate and polar body. J Assist Reprod Genet 40, 943–951 (2023). https://doi.org/10.1007/s10815-023-02758-3

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